Results 91 to 100 of about 517,584 (294)

Phosphoproteins and protein-kinase activity in isolated envelopes of pea (Pisum sativum L.) chloroplasts [PDF]

, 1985
A protein kinase was found in envelope membranes of purified pea (Pisum sativum L.) chloroplasts. Separation of the two envelope membranes showed that most of the enzyme activity was localized in the outer envelope.
D.G. Robinson, G. Mourioux, G.M. Hathaway, H.Y. Nakatani, J. Joyard, J. Pfisterer, J. Soll, J. Soll, K. Cline, K. Cline, M.A. Block, O.H. Lowry, R. Douce, R. Douce, T.J. Yan, U.J. Flügge, W.A. Laing, Z.F. Lin   +17 more
core   +1 more source

Structural and functional analysis of Bacillus sarcosine oxidase and its activity toward cyclic imino acids

FEBS Open Bio, EarlyView.
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang, Yoshitaka Nakajima, Masae Kurobe, Tsutomu Nakamura, Tomoki Himiyama, Yoshiaki Nishiya   +5 more
wiley   +1 more source

A rapid 3% polyacrylamide slab gel electrophoresis method for high through put screening of LDL phenotype

Lipids in Health and Disease, 2008
Background Small dense LDL is reported to be associated with increased coronary artery disease risk by various epidemiological studies. The gold standard for separation and identification of LDL subtypes in plasma is ultracentrifugation which is a ...
Gupta Ruby, Lakshmy Ramakrishnan, Singh Yogendra, Kranthi Vemparala   +3 more
doaj   +1 more source

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE

Bio-Protocol, 2018
The hierarchical composition and interactions of the labile thylakoid protein complexes can be assessed by sequential 2D-native gel-electrophoresis system.
Marjaana Rantala, Virpi Paakkarinen, Eva-Mari Aro   +2 more
doaj   +1 more source

Purification and characterization of very low density lipoprotein in commercial broiler and crossbred village chickens by fast protein liquid chromatography [PDF]

, 2005
Very low density lipoprotein (VLDL) of commercial broiler (CB) and crossbred village chicken (AK) was purified using Fast Protein Liquid Chromatography (FPLC).
Abdullah, Norhani, Foo, Hooi Ling, Idrus, Zulkifli, Loh, Teck Chwen, Tan, Bee Koon   +4 more
core   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Antigenic profile of heat-killed versus thimerosal-treated Leishmania major using sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Advanced Biomedical Research, 2015
Background: Leishmania is a parasitic protozoan of trypanosomatidae family which causes a wide spectrum of diseases ranging from self-healing cutaneous lesions to deadly visceral forms.
Reza Arjmand, Simindokht Soleimani Fard, Sedigheh Saberi, Sepideh Tolouei, Ali Khamesipour, Seyed Hossein Hejazi   +5 more
doaj   +1 more source

Importance of geometry of the extracellular matrix in endochondral bone differentiation. [PDF]

, 1984
Subcutaneous implantation of coarse powders (74-420 micron) of demineralized diaphyseal bone matrix resulted in the local differentiation of endochondral bone.
Reddi, AH, Sampath, TK
core   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source