Results 101 to 110 of about 626,288 (358)

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE

open access: yes, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Kastenholz, B.   +3 more
core   +1 more source

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

open access: yesAdvanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang   +13 more
wiley   +1 more source

Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae

open access: yes, 1989
Lipopolysaccharide (LPS) extracts obtained from Treponema hyodysenteriae of serogroups A, B, D and E, and from T. innocens were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit ...
Combs, B., Hampson, D.J., Mhoma, J.R.L.
core  

Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms

open access: yesAdvanced Science, EarlyView.
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu   +12 more
wiley   +1 more source

Distribution of Gc Types in Wuhan [PDF]

open access: yesFayixue Zazhi, 1985
The distribution of group-specific component(G) types was examined by disc electrophoresis from 508 unrelated residents in Wuhan. Discontinuous buffer system was used in this investigation. The polyacrylamide concentration used for separating gel was 9%,
LONG YANG YANG QING-EN(DEPARTEMENT OF FORENSIC MEDICINE;WUHAN MEDICAL COLLEGE;WUHAN)
doaj  

Urinary cholesterol: its association with a macromolecular protein- lipid complex [PDF]

open access: yes, 1984
The cholesterol-containing complexes in the urine of normal subjects and patients with diseases accompanied by hyperexcretion of urinary cholesterol were characterized. In normal subjects, the major portion of the recovered urinary cholesterol was eluted
Jüngst, Dieter   +3 more
core  

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

open access: yesAdvanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Reuse of Denaturing Polyacrylamide Gels for Short Tandem Repeat Analysis

open access: yesBioTechniques, 1998
Denaturing polyacrylamide gel electrophoretic analysis of amplified polymorphic short tandem repeat (STR) loci using fluorescent markers is a mainstay of forensic and paternity testing.
Allan Tereba   +2 more
doaj   +1 more source

GATA4‐Driven Transcription of HtrA1 Promotes Cellular Senescence in Ménière's Disease and Age‐Related Audio‐Vestibular Dysfunction

open access: yesAdvanced Science, EarlyView.
This study identifies the HDAC6/GATA4/HtrA1 axis as a critical driver of cellular senescence in the inner ear. GATA4 nuclear translocation, facilitated by HDAC6 downregulation, transcriptionally activates HtrA1, promoting hair cell senescence, SASP, and audio‐vestibular dysfunction in models of Ménière's disease and age‐related audio‐vestibular ...
Na Zhang   +16 more
wiley   +1 more source

Preparation of a polyacrylamide gel

open access: yes, 2021
For the separation of proteins by electrophoresis in polyacrylamide gel, once the system where to polymerize the gel is already assembled, the gel will be prepared, as explained in this video.https://media.upv.es/player/?id=326f3190-8273-11eb-9c0f ...
Yenush, Lynne, Andrés-Colás, Nuria
core  

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