Results 121 to 130 of about 626,288 (358)
Advanced Science, EarlyView.ABSTRACT Liver metastasis is a leading cause of mortality in colorectal cancer (CRC), where the inflammatory tumor microenvironment, specifically neutrophil infiltration, significantly promotes metastatic colonization. This study reveals a pro‐metastatic role for alpha‐1 antitrypsin (A1AT) in CRC liver metastasis via a dual mechanism involving ...
Qian Fei +11 more
wiley +1 more source
The construction of a secretary eukaryotic expression plasmid of chondroitinase ABC Ⅰ
Chinese Journal of Contemporary Neurology and Neurosurgery, 2010 Objective To construct a secretary eukaryotic expression plasmid for chondroitinase ABCⅠ, and observe the expression of the target protein in a glioma cell line TJ905. Methods The DNA fragments encoding the signal peptide of basilar membrane 40 (BM40) as
Hui⁃ling GAO +5 more
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, 1987 The hypothesis was tested that polyacrylamide gel which is presumably of constant fiber structure within a practical range of gel concentrations would yield a linear Ferguson plot in the gel electrophoresis of particles in the size range of 10–100 nm ...
Tietz, D., Orbán, L., Chrambach, A.
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Polyacrylamide Gel Electrophoresis of RNA: Figure 1.
, 2010 INTRODUCTIONPerhaps the most important and certainly the most often used technique in RNA analysis is gel electrophoresis. This technique is generally applicable for RNA detection, quantification, purification by size, and quality assessment.
Gregory J. Hannon +3 more
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Advanced Science, EarlyView.Monoclonal antibodies are powerful anticancer therapeutics targeting tumor‐associated antigens. Mass spectrometry enables precise and accurate quantification of mAbs in tumor plasma and healthy organs. Unlike ligand binding assay and radioactivity, mass spectrometry does not suffer from matrix effects and labelling artefacts while facilitating method ...
Domenico Ravazza +9 more
wiley +1 more source
Purification of DNA oligos-denaturing polyacrylamide gel electrophoresis (PAGE).
, 2015 After chemical synthesis, the oligonucleotide preparation contains the desired full-length oligonucleotide but also all of the DNA molecules that were aborted during each cycle in the synthesis, and the by-products generated during the chemical reactions.
López-Gomollón, Sara, Nicolas, F. E.
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Caracterização proteômica de possíveis efeitos pleiotrópicos em milho geneticamente modificado (MON810) [PDF]
, 2011 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias, Programa de Pós-Graduação em Recursos Genéticos Vegetais, Florianópolis, 2011Estudos de biossegurança que comparam OGMs e não-OGMs geralmente compreendem ...
Agapito-Tenfen, Sarah Zanon
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Polyacrylamide Gel Electrophoresis of Human Salivary Histidine-Rich-Polypeptides
, 1977 A method of polyacrylamide gel electrophoresis for examining histidine-rich-polypeptides in human saliva is described. Comparison is made to several commonly used electrophorretic techniques.
Janice L. Bird +2 more
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PSMA8‐Containing 20S Proteasome Regulates Spermiogenesis and Male Fertility
Advanced Science, EarlyView.PSMA8 assembles s20S proteasome that degrades specific substrates in elongating spermatids. Degradation of s20S‐substrates activates translation of FXR1‐target mRNAs, which are essential for mitochondrial sheath formation and sperm morphogenesis.
Huiwen Cao +7 more
wiley +1 more source
A Simpler and Faster Version of Two-Dimensional Gel Electrophoresis Using Vertical, Mini SDS-PAGE Apparatus [PDF]
Iranian Journal of Chemistry & Chemical Engineering, 2009 We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods.
Yaser Dorri +2 more
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