Results 261 to 270 of about 517,584 (294)

Polyacrylamide gel electrophoresis on micro slabs

Analytical Biochemistry, 1972
Abstract An electrophoretic technique using micro polyacrylamide flat gels is described and its usefulness demonstrated. The gels are vertically cast and electrophoresed in slab form (75 × 18 × 0.75 mm) in closed thin glass cells (cuvets) made from detachable microscope slides.
H. Rainer Maurer, Francesco A. Dati
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SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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High concentration polyacrylamide gel electrophoresis

Analytical Biochemistry, 1986
A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
H.G. Vartak, Manjusri Dutta Choudhury, Vandana S. Dhamankar   +2 more
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Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

Analytical Biochemistry, 1969
Abstract Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.
Elliot L. Elson, Thomas M. Jovin
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Discontinuous polyacrylamide gel electrophoresis of RNA

Analytical Biochemistry, 1972
Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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SDS – polyacrylamide gel electrophoresis of lipopolysaccharides

Canadian Journal of Microbiology, 1975
Lipopolysaccharides (LPS) prepared from four different species of Neisseria have been separated by SDS – polyacrylamide gel electrophoresis. Each LPS possessed a characteristic mobility on gels. Examination of the effect of acrylamide concentration on migration illustrated that the basis of the separation was molecular size, and not intrinsic charge.
K. G. Johnson, R. R. B. Russell
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A miniaturized system for electrophoresis on polyacrylamide gels

Analytical Biochemistry, 1979
The design, construction, and use of a simple economical system for gel electrophoresis are described, along with effective procedures and the required materials. Examples of successful use of the system are presented.
Clement L. Markert, Zen-ichi Ogita
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Electrophoresis of polysaccharides in a polyacrylamide gel

Bulletin of the Academy of Sciences of the USSR Division of Chemical Science, 1969
A metod for the electrophoresis of acid and neutral polysaccharides in polyacrylamide gel is described.
A. F. Pavlenko, Yu. S. Ovodov
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Disc Electrophoresis in Polyacrylamide Gel

1979
Earlier electrophoretic procedures, such as paper electrophoresis described in Exercise No. 9 of this Manual, were rapidly replaced by the much better resolving electrophoresis procedures in gel. While regular paper or cellulose acetate paper electrophoresis detected a maximum of 6 to 7 components in a human serum sample, electrophoresis in ...
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Polyacrylamide Temperature Gradient Gel Electrophoresis

2013
Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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