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Micro-Electrophoresis on Polyacrylamide Gels
1973Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
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Discontinuous polyacrylamide gel electrophoresis of RNA
Analytical Biochemistry, 1972Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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Neutral Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).
Methods in Enzymology, 2014J. Brunelle, R. Green
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RNA purification by preparative polyacrylamide gel electrophoresis.
Methods in Enzymology, 2013A. Petrov +3 more
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