Results 41 to 50 of about 705,442 (212)
Static Virus Spread Algorithm for DNA Sequence Design [PDF]
arXiv, 2023 DNA is not only the genetic material of life, but also a favorable material for a new computing model. Various research works based on DNA computing have been carried out in recent years. DNA sequence design is the foundation of such research. The sequence quality directly affects the universality, robustness, and stability of DNA computing.
arxiv
Silver staining of proteins in polyacrylamide gels [PDF]
Nat Protoc 1, 4 (2006) 1852-8, 2007 Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of
arxiv +1 more source
Türk Biyokimya Dergisi, 2022 In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat +3 more
doaj +1 more source
How to use 2D gel electrophoresis in plant proteomics [PDF]
Methods in Molecular Biology -Clifton then Totowa- 1072 (2014)
43-50, 2013 Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays.Nevertheless, 2D gel-based proteomics still has valuable features, and sometimes unique ones, which make it often an ...
arxiv +1 more source
Optimization of microsatellite DNA Gelred fluorescence imaging technology [PDF]
, 2016 Gelred fluorescent dye has broader prospects of application in DNA experiment because of its high sensitivity, security and stability. In order to explore the best microsatellite DNA Gelred imaging technology, this study compared its dosage by using ...
Da-li, Fu +4 more
core +2 more sources
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Bio-Protocol, 2012 One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
doaj +1 more source
A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1. [PDF]
PLoS ONE, 2017 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research.
Rahul Mahadev Shelake +4 more
doaj +1 more source
DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS [PDF]
Poljoprivreda, 2011 Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated.
Ivica Strelec +2 more
doaj
The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
Evans, RW, Williams, J
core +2 more sources
Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients [PDF]
Electrophoresis 18 (31/03/1997) 307-16, 2006 Membrane and nuclear proteins of poor solubility have been separated by high resolution two-dimensional (2-D) gel electrophoresis. Isoelectric focusing with immobilized pH gradients leads to severe quantitative losses of proteins in the resulting 2-D map, although the resolution is usually high.
arxiv +1 more source