Holliday junction resolvase in Schizosaccharomyces pombe has identical endonuclease activity to the CCE1 homologue YDC2 [PDF]
, 1998 A novel Holliday junction resolving activity has been identified in fractionated cell extracts of the fission yeast Schizosaccharomyces pombe . The enzyme catalyses endonucleolytic cleavage of Holliday junction-containing chi DNA and synthetic four-way ...
Keeley, A, Oram, M, Tsaneva, IR
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Acta Societatis Botanicorum Poloniae, 2015 SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and ...
Aleksander F. Sikorski
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BMC Research Notes, 2021 Objective The advancement of molecular techniques in an era in which high-throughput sequencing has revolutionized biology renders old-fashioned alternatives to high-throughput methods obsolete.
Julissa J. Sánchez-Velásquez +3 more
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A simple method of drying polyacrylamide slab gels that eliminates cracking
BioTechniques, 2021 The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
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Characterization of the Lipopolysaccharide from a \u3cem\u3eRhizobium phaseoli\u3c/em\u3e Mutant that is Defective in Infection Thread Development [PDF]
, 1987 The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate ...
Carlson, Russell W. +4 more
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Use of capillary electrophoresis as a method development tool for classical gel electrophoresis [PDF]
, 1998 Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan.
Gratz, Samuel R. +2 more
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Detection of rotavirus genome by new silver staining method
مجله دانشگاه علوم پزشکی گرگان, 2014 Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
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Blue-native PAGE in plants: a tool in analysis of protein-protein interactions
Plant Methods, 2005 Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey +2 more
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The cell cycle program of polypeptide labeling in Chlamydomonas reinhardtii [PDF]
, 1977 The cell cycle program of polypeptide labeling in syndhronous cultures of wild-type Chlamydomonas reinhardtii was analyzed by pulse-labeling cells with 35SO4 = or [3H]arginine at different cell cycle stages.
Hill, Kim R. +2 more
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Elution of proteins from polyacrylamide eels: a simple and economic procedure
Revista do Instituto de Medicina Tropical de São Paulo, 1989 A simplified methodology for the quantitative electroelution of proteins from polyacrylamide gels is described. After staining with Coomassie Brilliant Blue R 250, the identified bands are excised from the gel and the proteins eluted using a procedure ...
Vera Bongertz
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