Results 111 to 120 of about 1,070,541 (305)

Determination of nucleosides natural Cordyceps sinensis and cultured Cordyceps mycelia by capillary electrophoresis

, 2001
By transforming the time-based x-axis of electropherograms in capillary zone electrophoresis (CZE) into the corresponding effective mobility-scale, we propose a simple and robust data representation for a better qualitative and quantitative capillary ...
Pruijn, G J M,, Craft, Joe,, Seidl, R, Tsim, Karl W.K., Achsel, Tilmann,, Pruijn, GJM, Dong, Tina T.X., Achsel, T, Lubec, Gert,, Cairns, N, Craft, J, Li, Shao P., Henriksson, Elisabet Welin,, Bidmon, Bettina,, Turhani-Schatzmann, D, Bidmon, B, Labudova, O, Li, Ping, Cairns, Nigel,, Seidl, Rainer,, Labudova, Olga,, Krapfenbauer, Kurt,, Henriksson, EW, Turhani-Schatzmann, Dritan,, Lubec, G   +24 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Microparticles for selective protein determination in capillary electrophoresis

, 2001
A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a
Nilsson, S., Nilsson, H., Johansson, T., Hertz, Hans M.,, Wiklund, Martin,   +4 more
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

High-voltage power supplies to capillary and microchip electrophoresis

, 2012
Over the past years, the development of capillary electrophoresis (CE) and microchip electrophoresis (ME) systems has grown due to instrumental simplicity and wide application.
Claudimir Lucio do Lago, Van Gramberg, A, Doble, P, Saito, RM, Wendell Karlos Tomazelli Coltro, Lucio do Lago, C, Renata Mayumi Saito, Blanes, L, Lucas Blanes, Amanda Van Gramberg, Coltro, WKT, Philip Doble   +11 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Development of a universal serial bus-powered mini-high-voltage power supply for microchip electrophoresis

, 2007
We describe a miniature high-voltage power supply (HVPS) with dimensions of 4.7 x 5.6 x 2.5 cm (W x L x H) powered by universal serial bus (USB) ports. Two strategies were adopted to ensure its efficient power usage. (i) Only two high-voltage converters (
Qin, Jianhua, 林炳承, Dai, Zhongpeng, Xie, Minhao, Lu, Yao, Jiang, Lei, Jiang, Xuening, Lin, Bingcheng   +7 more
core   +1 more source

Capillary electrophoresis, a new tool in hair analysis for abused drugs

, 1997
Capillary electrophoresis, a new tool in hair analysis for abused ...
Manetto G., TURRINA, Stefania, MARIGO, Mario, Crivellente F., TAGLIARO, Franco, PISI, Paolo   +5 more
core   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source