Results 101 to 110 of about 1,000,843 (316)

Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2). [PDF]

, 2009
BACKGROUND: Genotyping of Plasmodium falciparum based on PCR amplification of the polymorphic genes encoding the merozoite surface proteins 1 and 2 (msp1 and msp2) is well established in the field of malaria research to determine the number and types of ...
Färnert, Anna, Kweku Margaret, Mårtensson, Andreas, Margaret Kweku, Mårtensson, A., Felger Ingrid, Felger, Ingrid, Mårtensson Andreas, Kweku, M., Liljander Anne, Ingrid Felger, Falk, Nicole, Farnert, A., Kweku, Margaret, Lisa Wiklund, Andreas Mårtensson, Felger, I., Anna Färnert, Liljander, A., Wiklund Lisa, Liljander, Anne, Falk Nicole, Anne Liljander, Wiklund, L., Falk, N., Färnert Anna, Wiklund, Lisa, Nicole Falk   +27 more
core   +1 more source

Fluid Dynamics and Electrical Detection of λDNA in Electrode-Embedded Nanochannels

Journal of Biomechanical Science and Engineering, 2013
In the present study, we address theoretical approaches for the experimental results to investigate the flow dynamics of λDNA through a nanochannel in which two nanoelectrodes are integrated.
Satoshi UEHARA, Makusu TSUTSUI, Kentaro DOI, Masateru TANIGUCHI, Satoyuki KAWANO, Tomoji KAWAI   +5 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Further improvement of hydrostatic pressure sample injection for microchip electrophoresis [PDF]

, 2007
Hydrostatic pressure sample injection method is able to minimize the number of electrodes needed for a microchip electrophoresis process; however, it neither can be applied for electrophoretic DNA sizing, nor can be implemented on the widely used single ...
Qin, Jianhua, Luo, Yong, Zhang, Qingquan, Lin, Bingcheng   +3 more
core   +1 more source

Coupling gel electrophoresis with photoluminescence reveals biochrome complexes in modern and fossil shells

Methods in Ecology and Evolution
Polyacrylamide gel electrophoresis is commonly used to visualize mixtures of proteins, such as those embedded in calcareous biominerals. However, it is ineffective for the detection of biochromes, a major class of low molecular weight organic compounds ...
Luca Polacchi, Camille Lutet‐Toti, Olivier Basuyaux, Pierre Gueriau, Marie Albéric, Hélène Pasco, Jérôme Thomas, Didier Merle, Benoît Habermeyer, Roger Guilard, Mathieu Thoury, Frédéric Marin   +11 more
doaj   +1 more source

Genetic diversity of kaffir sorghum accessions from the VIR collection in kafirin-encoding loci

Труды по прикладной ботанике, генетике и селекции
Background. Sorghum is a universal, highly drought-resistant crop, widely cultivated throughout the world. Sorghum grain has a valuable biochemical composition and is utilized in both food and feed production.
N. V. Alpatieva, I. N. Anisimova, M. K. Ryazanova, V. V. Vasipov, R. A. Abdullaev, O. I. Romanova, E. E. Radchenko   +6 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Two-dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins [PDF]

, 2015
Two-dimensional electrophoresis of Cereus peruvianus callus tissues grown in culture media containing two different 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin combinations was used to identify minor differences in polypeptide composition of these
Mangolin, CA, Ottoboni, LMM, Machado, MDPS   +2 more
core   +1 more source

The determination of isoflavones and coumestrol by capillary electrophoresis

Czech Journal of Food Sciences, 2001
The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DAD
J. Moravcová, T. Kleinová
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source