Results 121 to 130 of about 1,070,541 (305)

Enantiomer Separations by Capillary Electrophoresis

, 2016
Click on the DOI link to access the article May not be free).Capillary electrophoresis (CE) is a versatile and flexible technique for analytical enantioseparations.
Henrik Harnisch, Scriba, Gerhard K. E., Qingfu Zhu, Harnisch, Henrik, Zhu, Qingfu, Gerhard K. E. Scriba   +5 more
core   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

The ratio of structural proteins in the wool of sheep of different breeds and with different categories of fibers depending on the zones of their breeding

Біологія тварин
Wool fiber consists of a complex of protein-keratins, which differ in both their physical and chemical properties and functional characteristics. Despite the progress achieved in the study of the structural organization of fibers, the issue of the ...
P. V. Stapay, T. V. Buslyk, V. V. Mykhaliuk, N. P. Stakhiv, O. O. Smolyaninova, I. V. Nevostruyeva, R. G. Sachko, А. V. Skorokhid, N. I. Pakholkiv   +8 more
doaj   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Two-dimensional electrophoresis of Cereus peruvianus (Cactaceae) callus tissue proteins

, 2015
Two-dimensional electrophoresis of Cereus peruvianus callus tissues grown in culture media containing two different 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin combinations was used to identify minor differences in polypeptide composition of these
Mangolin, CA, Ottoboni, LMM, Machado, MDPS   +2 more
core   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Investigation of genetic structure of angora goat by using starch gel electrophoretic method [PDF]

Eurasian Journal of Veterinary Sciences
In this present study, we determined the genetic structure of Angora and other breeds of goats. For this purpose, five different enzyme loci; superoxide dismutase (SOD), carbonic anhydrase 1 (CA1), esterase D (EsD), glutamic oxaloasetic transaminase (GOT)
Vahdettin Altunok, Mehmet Nizamlıoğlu, Zafer Bulut   +2 more
doaj  

Percent Pulmonary Damage on Hospital Admission is Predictive of COVID-19 Aggravation, but Serum Protein Electrophoresis is Not

Journal of Global Infectious Diseases
Introduction: Electrophoresis of serum proteins (ESP) reveals the proteins present during the acute phase of a disease and enables the detection and monitoring of inflammatory syndrome.
Stéphane Di Mascio, Fadi Khadra, Christine Tchikladzé-Mérand, Sandrine Labroy, Mélanie Decambron   +4 more
doaj   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source