Results 121 to 130 of about 1,000,843 (316)
COMPARATIVE STUDY OF AUTOLYTIC CHANGES IN PORK AND BEEF MUSCLE TISSUE PROTEOME
Теория и практика переработки мяса, 2018 The paper presents the studies on the autolytic transformations in pork and beef samples by proteomic methods. The changes in the protein fractions were analyzed by one-dimensional and two- dimensional electrophoresis with the following identification by
Irina M. Chernukha +1 more
doaj +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Capillary electrophoresis: A new methodological approach to the analysis of molecules and a challenge for biochemists [PDF]
Vojnosanitetski Pregled, 2003 Žunić Gordana
doaj +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Electrophoresis of biological cells: charge-regulation and multivalent counterions association model [PDF]
, 2009 The electrophoresis of a biological cell is analyzed theoretically. An entity, which is of amphoteric nature, is used to simulate its electrophoretic behavior. To reflect conditions of practical interest, we assume that the liquid phase contains mixed (a:
Hsu, Jyh-ping; Hseih, Tien-Shiang ; Young, Tai-Horng; 曾琇瑱; Tseng, Shio-jenn +1 more
core +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
The application of capillary electrophoresis and liquid chromatography/mass spectrometry to the analysis of anaerobic and cyanoacrylate adhesives [PDF]
, 2002 This thesis details the application of separation techniques to the analysis of anaerobic and cyanoacrylate adhesives Chapter 1 is an introduction to adhesives, where the chemistry, composition and analysis of both types of adhesives are discussed ...
McCullagh, Niamh M.
core
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Chip electrophoresis, Fluorescence, Gelatin, Lab-on-a-chip, Nanoparticle [PDF]
, 2013 ecently, biodegradable nanoparticles receive increasing attention for pharmaceutical applications as well as applications in food industry. With the current investigation we demonstrate chip electrophoresis of fluorescently (FL) labeled gelatin ...
Lehner, Angela +4 more
core
FEBS Open Bio, EarlyView.The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source