Results 81 to 90 of about 1,070,541 (305)

Immunoaffinity Capillary Electrophoresis: Determination of Binding Constant and Stoichiometry for Antibody-Antigen Interaction

, 2009
Affinity capillary electrophoresis (ACE) can provide both qualitative and quantitative information on molecular interactions and affords the advantages of very low sample consumption, high mass sensitivity, short analysis time, and the use of automated ...
陳瑞堅;林世明, CHEN, RUEY-JIEN;LIN, SHI-MING   +1 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Application of capillary nongel sieving electrophoresis for gene analysis

, 1999
Capillary electrophoresis (CE) has proved to be a strong tool for DNA analysis and has found abundant applications in the fields of restriction fragment sizing, mutation screening, polymerase chain reaction (PCR) product characterizing and forensic ...
Zhu, N, Song, F, Shen, Y, Ding, K, Xu, Q, Han, FT, Fan, Y, Wu, GY, Lin, BC   +8 more
core  

Fluid Dynamics and Electrical Detection of λDNA in Electrode-Embedded Nanochannels

Journal of Biomechanical Science and Engineering, 2013
In the present study, we address theoretical approaches for the experimental results to investigate the flow dynamics of λDNA through a nanochannel in which two nanoelectrodes are integrated.
Satoshi UEHARA, Makusu TSUTSUI, Kentaro DOI, Masateru TANIGUCHI, Satoyuki KAWANO, Tomoji KAWAI   +5 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Enantiomer separation of disopyramide with capillary electrophoresis using various cyclodextrins

, 1997
Enantiomers of disopyramide display different biological actions, and therefore chiral selective analysis is necessary. Fifteen different cyclodextrins (CDs) and CD derivatives were tested as capillary electrophoresis (CE) additives for the chiral ...
Markides, KE, Juvancz, Z,, Jicsinszky, L
core  

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Coupling gel electrophoresis with photoluminescence reveals biochrome complexes in modern and fossil shells

Methods in Ecology and Evolution
Polyacrylamide gel electrophoresis is commonly used to visualize mixtures of proteins, such as those embedded in calcareous biominerals. However, it is ineffective for the detection of biochromes, a major class of low molecular weight organic compounds ...
Luca Polacchi, Camille Lutet‐Toti, Olivier Basuyaux, Pierre Gueriau, Marie Albéric, Hélène Pasco, Jérôme Thomas, Didier Merle, Benoît Habermeyer, Roger Guilard, Mathieu Thoury, Frédéric Marin   +11 more
doaj   +1 more source

Genetic diversity of kaffir sorghum accessions from the VIR collection in kafirin-encoding loci

Труды по прикладной ботанике, генетике и селекции
Background. Sorghum is a universal, highly drought-resistant crop, widely cultivated throughout the world. Sorghum grain has a valuable biochemical composition and is utilized in both food and feed production.
N. V. Alpatieva, I. N. Anisimova, M. K. Ryazanova, V. V. Vasipov, R. A. Abdullaev, O. I. Romanova, E. E. Radchenko   +6 more
doaj   +1 more source

The determination of isoflavones and coumestrol by capillary electrophoresis

Czech Journal of Food Sciences, 2001
The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DAD
J. Moravcová, T. Kleinová
doaj   +1 more source