Results 61 to 70 of about 996,335 (316)

Mechanism of reducing coal powder sedimentation by electrophoresis in coalbed methane drainage

open access: yesMeitan xuebao
Coal powder blockage seriously affects the efficient development of coalbed methane, and existing blockage removal methods have problems such as low efficiency and high cost. Therefore, based on the charged characteristics of coal in aqueous solution, it
Junqing GUO   +5 more
doaj   +1 more source

Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

open access: yesPlant, Soil and Environment, 2003
In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins ...
T. Vyhnánek, J. Bednář
doaj   +1 more source

Microsecond electrophoresis [PDF]

open access: yesProceedings of the National Academy of Sciences, 2003
Although analysis strategies exist for probing a diverse array of molecular properties, most of these approaches are not amenable to the study of reaction intermediates and other transient species. Separations in particular can provide detailed information on attributes not readily measured by spectroscopy but typically are performed over ...
Matthew L, Plenert, Jason B, Shear
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Gel electrophoresis of a charge-regulated, bi-functional particle

open access: yes, 2014
Adopting a Brinkman fluid model, we analyzed the electrophoresis of a charged-regulated, bi-functional particle containing both acidic and basic functional groups in a gel solution. Both the long-range hydrodynamic effect arising from the liquid drag and
Hsu, Jyh-Ping; Huang, Chih-Hua; Tseng, Shiojenn   +1 more
core   +1 more source

Methods of gadoid fish species identification in food and their economic impact in the Czech Republic: a review

open access: yesVeterinární Medicína, 2007
The purpose of the present study was to give an overview of imported and traded gadoid fish species (Gadiformes) in the Czech Republic and to describe available methods for their authentication. Due to the increasing interest of customers in the purchase
Z. Hubalkova   +3 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Serum proteins and their diagnostic utility in veterinary medicine: a review

open access: yesVeterinární Medicína, 2016
The evaluation of serum proteins and their electrophoretic pattern is a well-established laboratory method in the diagnosis of many diseases in humans, which has replaced the biochemical determination of the concentrations of albumin and the ratio of ...
C. Tothova, O. Nagy, G. Kovac
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Immunoaffinity Capillary Electrophoresis: Determination of Binding Constant and Stoichiometry for Antibody-Antigen Interaction

open access: yes, 2009
Affinity capillary electrophoresis (ACE) can provide both qualitative and quantitative information on molecular interactions and affords the advantages of very low sample consumption, high mass sensitivity, short analysis time, and the use of automated ...
陳瑞堅;林世明   +1 more
core  

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