Single-molecule measurements of trapped and migrating circular DNA during electrophoresis in agarose gels [PDF]
, 2006 The effect of agarose gel concentration and field strength on the electrophoretic trapping of open (relaxed) circular DNA was investigated using microscopic measurements of individual molecules stained with a fluorescent dye.
Gaigalas, A. +6 more
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Molecular Oncology, EarlyView.Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla +9 more
wiley +1 more source
Applications of homochiral metal-organic frameworks in enantioselective adsorption and chromatography separation [PDF]
, 2014 Chiral separation is of great importance for drug development, pharmacology, and biology. Chiral metal-organic frameworks (MOFs) is a new class of porous solid materials with high surface area, large pore size, high chemical stability, uniformly ...
Liu, Huwei +4 more
core +1 more source
CCDC80 suppresses high‐grade serous ovarian cancer migration via negative regulation of B7‐H3
Molecular Oncology, EarlyView.PAX8 is a lineage‐specific master regulator of transcription in high‐grade serous ovarian cancer (HGSC) progression. We show for the first time that PAX8 facilitates proliferation and metastasis by repressing the cell autonomous tumor suppressor CCDC80 and inducing B7‐H3 expression.
Aya Saleh +12 more
wiley +1 more source
Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique [PDF]
, 2011 Jasmonic acid (JA), an essential plant hormone controlling the plant defense signaling system and developmental processes, has stereospecific bioactivities that have not been well understood mainly due to the limitation in separation and detection ...
Liu, Huwei +7 more
core +1 more source
Пищевые системы, 2023 The Vitis vinifera L. UFGT gene is one of the diagnostically significant genes for genetic testing of technical grape varieties as well as wine materials and wines produced from them.
R. R. Vafin +2 more
doaj +1 more source
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
Using Affinity Capillary Electrophoresis to Evaluate Average Binding Constant of 18-Mer Diphospho-Tyrosine Peptide to Antiphosphotyrosine Fab [PDF]
, 2009 We used affinity electrophoresis in capillaries to investigate the interaction between a monovalent antiphosphotyrosine antibody fragment, antigen-binding fragment (Fab), and a divalent antigen (dAg), an 18-mer diphosphopeptide phosphorylated on two-site
Lin, Shiming; Tang, Petrus; Hsu, Su-Ming +1 more
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Application of capillary electrophoresis to amino acid sequencing of peptide [PDF]
, 2021 Separations of twenty phenylthiohydantoin (PTH) amino acids, and amino acid derivatives, resulting from the Edman degradation of peptides and proteins, were optimized for peptide sequencing by capillary electrophoresis.
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