Microchip Free-flow Electrophoresis On Glass Substrate Using Laser-printing Toner As Structural Material [PDF]
, 2015 In this work, a microfluidic free-flow electrophoresis device, obtained by thermal toner transferring on glass substrate, is presented. A microdevice can be manufactured in only 1 h.
de Jesus D.P., Bianes L., do Lago C.L.
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Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
A Simple, Rapid, and Sensitive Method for Analysis of SYPRO Red Labeled SDS-Protein Complexes by Capillary Electrophoresis with Laser-Induced Fluorescence [PDF]
, 2010 [[abstract]]We describe a segmental filling method for the analysis of SYPRO Red labeled sodium dodecyl sulfate (SDS)-proteins (SRSPs) by capillary electrophoresis-laser induced fluorescence (CE-LIF) with electroosmotic counterflow of poly(ethylene oxide)
Chiu, T.-C.; Lin, Y.-W.; Huang, C.-C.; Chrambach, A.; Chang, H.-T.
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Microparticles for selective protein determination in capillary electrophoresis [PDF]
, 2001 A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a
Nilsson, S. +4 more
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An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ [PDF]
, 2011 A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow ...
Bucci, Em +7 more
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Rationale for the use of electrophoresis of drugs in post-stroke patients with visual impairments: a review [PDF]
Вестник восстановительной медициныINTRODUCTION. Disorders of vision and oculomotor functions are widespread among patients who have suffered a cerebral stroke. The rehabilitation system for patients who have suffered a stroke makes use of physiotherapy methods that can significantly ...
Detelina B. Kulchitskaya +3 more
doaj +1 more source
Micromachines, 2022 Initial screening of criminal evidence often involves serological testing of stains of unknown composition and/or origin discovered at a crime scene to determine the tissue of origin. This testing is presumptive but critical for contextualizing the scene.
Tiffany R. Layne +4 more
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Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source