Results 71 to 80 of about 996,335 (316)

Microchip Free-flow Electrophoresis On Glass Substrate Using Laser-printing Toner As Structural Material

open access: yes, 2015
In this work, a microfluidic free-flow electrophoresis device, obtained by thermal toner transferring on glass substrate, is presented. A microdevice can be manufactured in only 1 h.
de Jesus D.P., Bianes L., do Lago C.L.
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

open access: yesFEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel   +4 more
wiley   +1 more source

Fractionnement et caractérisation des glycoprotéines dans les moûts de raisins blancs

open access: yesOENO One, 1990
Les auteurs étudient les glycoprotéines dans des moûts de raisins blancs. Ces glycoprotéines sont fractionnées par chromatofocalisation et caractérisées par différentes techniques séparatives : CLHP, électrophorèse, SDS-PAGE, analyse des acides aminés ...
Michael Paetzold   +2 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

A Simple, Rapid, and Sensitive Method for Analysis of SYPRO Red Labeled SDS-Protein Complexes by Capillary Electrophoresis with Laser-Induced Fluorescence

open access: yes, 2010
[[abstract]]We describe a segmental filling method for the analysis of SYPRO Red labeled sodium dodecyl sulfate (SDS)-proteins (SRSPs) by capillary electrophoresis-laser induced fluorescence (CE-LIF) with electroosmotic counterflow of poly(ethylene oxide)
Chiu, T.-C.; Lin, Y.-W.; Huang, C.-C.; Chrambach, A.; Chang, H.-T.
core  

Microparticles for selective protein determination in capillary electrophoresis

open access: yes, 2001
A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a
Nilsson, S.   +4 more
core   +1 more source

Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]

open access: yes, 1988
To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, K   +7 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

open access: yesFEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji   +2 more
wiley   +1 more source

Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ

open access: yes, 2011
A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow ...
Bucci, Em   +7 more
core   +1 more source

Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2). [PDF]

open access: yes, 2009
BACKGROUND: Genotyping of Plasmodium falciparum based on PCR amplification of the polymorphic genes encoding the merozoite surface proteins 1 and 2 (msp1 and msp2) is well established in the field of malaria research to determine the number and types of ...
Färnert, Anna   +27 more
core   +1 more source

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