Results 31 to 40 of about 1,414,906 (307)

Why Johnny can't clone: Common pitfalls and not so common solutions

BioTechniques, 2015
The demand for cloned genes has increased incessantly over the past 32 years, but some who need recombinant plasmids struggle to produce them. While the pitfalls of traditional ligation-dependent cloning are non-trivial, most can be avoided with ...
Ichiro Matsumura
doaj   +1 more source

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

Micromachines, 2021
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez, Francisco Perdigones, José Manuel Quero   +2 more
doaj   +1 more source

Re-use of commercial microfluidics chips for DNA, RNA, and protein electrophoresis

BioTechniques, 2014
Microfluidics chip technology is a powerful and convenient alternative to agarose gels and PAGE, but costs can be high due to certain chips being non-reusable.
Thi Nguyen, Sukyoung Kwak, Steven J. Karpowicz   +2 more
doaj   +1 more source

Proteomic Advances in Milk and Dairy Products

Molecules, 2021
Proteomics is a new area of study that in recent decades has provided great advances in the field of medicine. However, its enormous potential for the study of proteomes makes it also applicable to other areas of science.
Rubén Agregán, Noemí Echegaray, María López-Pedrouso, Radwan Kharabsheh, Daniel Franco, José M. Lorenzo   +5 more
doaj   +1 more source

DESIGN OF ELECTROPHORESIS DEVICE FOR OPTIMATION OF DNA VISUALIZATION AND DNA CONCENTRATION USING SOFTWARE

Jurnal Pendidikan Fisika Indonesia, 2014
Molekul DNA menunjukkan polarisasi yang kuat sehingga memungkinkan baik gerak elektroforesis berdasarkan muatan negatifnya maupun gerak dielektroforesis berdasarkan induksi polarisasi.
H.P. Kusumaningrum, W. S. Budi, M. Azam, A. Bawono   +3 more
doaj   +1 more source

Current Diagnostic Methods for Assessing Transfer of Passive Immunity in Calves and Possible Improvements: A Literature Review

Animals, 2021
Several direct or indirect methods can be used to assess immunoglobulin G (IgG) concentrations in calves, which evaluates the transfer of passive immunity (TPI).
Rayanne Soalheiro de Souza, Lucas Braga Costa dos Santos, Isabela Oliveira Melo, Daiane Maria Cerqueira, Juliana Vieira Dumas, Fabiola de Oliveira Paes Leme, Tiago Facury Moreira, Rodrigo Melo Meneses, Antônio Ultimo de Carvalho, Elias Jorge Facury-Filho   +9 more
doaj   +1 more source

Membrane proteins and proteomics: Love is possible, but so difficult [PDF]

, 2009
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi, Aebersold, Aivaliotis, Aivaliotis, Alla, Ames, Babu, Bagshaw, Bell, Berlanda-Scorza, Berven, Bierczynska-Krzysik, Bisle, Bjellqvist, Bunai, Bunai, Burré, Buxbaum, Casey, Chevallet, Chevallet, Connolly, Coughenour, Cutillas, Devreese, Eravci, Everberg, Felsted, Ferro, Gatlin, Ghosh, Gyenes, Görg, Hagan, Hahne, Hartinger, Helling, Henningsen, Henzel, Hisabori, Hjelmeland, Huang, Huang, James, Jun, Lasonder, Lescuyer, Liberatori, Lopez, Low, Luche, Lyubimova, Lüddens, MacFarlane, MacFarlane, Matsudaira, McDonough, Miguet, Moebius, Molloy, Molloy, Nandakumar, Natera, Navarre, Ong, Papasotiriou, Pappin, Patton, Pedersen, Peng, Perdew, Phadke, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Rais, Rubin, Santoni, Santoni, Schindler, Schirle, Schägger, Siroy, Speers, Sweetnam, Switzer, Tastet, Taylor, Towbin, Veith, Washburn, Werner, Wilkins, Williams, Williams, Williams, Wittig, Witzmann, Wu, Yates, Ying, Zahedi   +103 more
core   +5 more sources

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]

, 1993
The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
A.A. Friedl, A.M. Kellerer, Ager D.D., Ager D.D., Ahn S.Y., Andrews J., Blazek E.R., Blöcher D, Blöcher D, Blöcher D., Blöcher D., Blöcher D., Bronstein I., Contopoulou C.R., F. Eckardt-Schupp, Frankenberg D., Frankenberg-Schwager M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Geigl E.-M., Hanawalt P.C, Ho K.S.Y, Iliakis G.E., Iliakis G.E., Jacobs A., K. Hahn, Kessler B., Kunz B.A., Lennartz M., Okayasu R.B., Oleinick N.L., Radford I.R., Resnick M.A., Schulz G.V, Smith L.C., Stamato T.D., Terleth C., W. Beisker, Ward J.F   +39 more
core   +1 more source

An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: High-efficient fractionation and online mass spectrometry detection

Green Analytical Chemistry
We present an integrated protocol for studying the charge heterogeneity of therapeutic proteins combining two workflows: imaged capillary isoelectric focusing (icIEF) with fractionation and online mass spectrometry (MS) detection on a single platform ...
Teresa Kwok, She Lin Chan, Mike Zhou, Tong Chen, Niusheng Xu, Victor Li, Tiemin Huang, Tao Bo   +7 more
doaj   +1 more source

Retracted:An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: High-efficient fractionation and online mass spectrometry detection

Green Analytical Chemistry
This article has been retracted: Please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy).This article has been retracted at the request of the authors.
Teresa Kwok, Matt Countney, Tia Chan, Mike Zhou, Tong Chen, Victor Li, Tiemin Huang, Tao Bo   +7 more
doaj   +1 more source