Results 41 to 50 of about 1,414,906 (307)

Retraction notice to: An integrated protocol based on workflows of imaged capillary isoelectric focusing (icIEF) for in-depth protein heterogenous characterization: high-efficient fractionation and online mass spectrometry detection

open access: yesGreen Analytical Chemistry
This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/policies/article-withdrawal).This article has been retracted at the request of the authors.
Teresa Kwok   +7 more
doaj   +1 more source

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

open access: yes, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

open access: yesFEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto   +3 more
wiley   +1 more source

Low-cost modular systems for agarose gel documentation

open access: yesBioTechniques, 2022
While conducting recombinant DNA technology procedures, such as DNA purification, agarose gel electrophoresis is often used for identification, characterization and quantification of DNA.
Alfredo G Gonzalez   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Static continuous electrophoresis device [PDF]

open access: yes, 1982
An apparatus is disclosed for carrying out a moving wall type electrophoresis process for separation of cellular particles. The apparatus includes a water-tight housing containing an electrolytic buffer solution.
Rhodes, P. H.
core   +1 more source

Gel Electrophoresis

open access: yesClinical Chemistry, 1962
Abstract Gel electrophoresis is discussed and it is shown that this medium is still in the developmental stage for the resolution of serum proteins. It is the procedure of choice for the analysis of hemoglobins.
S, RAYMOND, M, NAKAMICHI
openaire   +2 more sources

Transcriptional network analysis of PTEN‐protein‐deficient prostate tumors reveals robust stromal reprogramming and signs of senescent paracrine communication

open access: yesMolecular Oncology, EarlyView.
Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice   +16 more
wiley   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

open access: yesMolecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova   +25 more
wiley   +1 more source

Rationale for the use of electrophoresis of drugs in post-stroke patients with visual impairments: a review [PDF]

open access: yesВестник восстановительной медицины
INTRODUCTION. Disorders of vision and oculomotor functions are widespread among patients who have suffered a cerebral stroke. The rehabilitation system for patients who have suffered a stroke makes use of physiotherapy methods that can significantly ...
Detelina B. Kulchitskaya   +3 more
doaj   +1 more source

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