Results 91 to 100 of about 192,083 (323)
Electroporation in Microfluidic Devices [PDF]
, 2016 Crossing the plasma cellular membrane for loading of exogenous substances or accessing the intracellular medium is essential for cell engineering and transfection, cell analysis, or controlled extraction of the cellular content. Various chemical and physical techniques have been developed to open up the cell membrane and allow molecular exchange ...
Le Gac, Séverine, van Uitert, Iris
openaire +3 more sources
Progress and Prospects of Persistent Luminescent Nanocrystals in Biomedical Applications
Advanced Optical Materials, EarlyView.This review summarizes recent progress in materials‐level control of persistent luminescence, including mechanistic understanding, wavelength/intensity tuning, and activation strategies, as well as emerging biomedical applications in imaging, biosensing, cell tracking, optogenetic stimulation, and biophotochemical activation.
Peng Pei +5 more
wiley +1 more source
Transfection by Electroporation [PDF]
Current Protocols in Cell Biology, 1992 AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
openaire +5 more sources
Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants
Advanced Science, EarlyView.In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao +12 more
wiley +1 more source
Advanced Science, EarlyView.This study identifies platelet‐derived extracellular vesicles as key immunometabolic regulators in COVID‐19. The delivery of tRF‐His‐GTG‐1 to neutrophils activates TLR8mTOR signaling, disrupts lipophagy, and amplifies NET‐mediated inflammation. Importantly, targeting this axis restores neutrophil homeostasis, offering a potential therapeutic strategy ...
Tsai‐Ling Liao +5 more
wiley +1 more source
Construction and utilisation of a bidirectional reporter vector in the analysis of two nod-boxes in of Rhizobium loti : a thesis presented in partial fulfilment of the requirements for the Degree of Master of Science in Molecular Genetics at Massey University [PDF]
, 1993 The nod-box is a 47bp cis-acting regulatory region which has been conserved amongst every species of Rhizobium studied to date. In species such as R. meliloti and R.
Parry, Simon Keith
core
Molecular Electroporation and the Transduction of Oligoarginines
, 2009 Certain short polycations, such as TAT and polyarginine, rapidly pass through the plasma membranes of mammalian cells by an unknown mechanism called transduction as well as by endocytosis and macropinocytosis.
Alberts B +20 more
core +1 more source
Advanced Science, EarlyView.A novel therapy using engineered immune cells (NAC‐T cells) showed promise for refractory malignant mesothelioma. Based on the encouraging preclinical data, the first‐in‐human trial is initiated, demonstrating tolerable safety and promising anti‐tumor activity (ORR 63.6%, DCR 100%, including one CR).
Yan Sun +23 more
wiley +1 more source
An Explorative Study on Calcium Electroporation for Low-risk Basal Cell Carcinoma
Acta Dermato-VenereologicaIn electrochemotherapy, permeabilization of the cell membrane by electric pulses increases the anti-tumour effect of chemotherapeutics. In calcium electroporation, chemotherapy is replaced by calcium chloride with obvious benefits.
Stine R. Wiegell +8 more
doaj +1 more source