Results 71 to 80 of about 135,255 (300)

Different cell viability assays following electroporation in Vitro

, 2018
ISBN 978-3-319-32885-0, 9783319267791Exposure of cells to high intensity electric fields can lead to death of some cells due to irreversible electroporation or due to electroporation-induced secondary effects.
Jakutavičiūtė, Milda, Jakštys, Baltramiejus, Ruzgys, Paulius, Šatkauskas, Saulius   +3 more
core   +1 more source

Dual‐Gene Edited Extracellular Vesicles Remodel the Redox Homeostasis to Inhibit Ferroptosis in Intervertebral Disc Degeneration

Advanced Science, EarlyView.
ABSTRACT Intervertebral disc degeneration (IDD) is driven by ferroptosis of nucleus pulposus cells (NPCs) as a core pathological mechanism. Nucleus pulposus progenitor cells (NPPCs), exhibiting stem cell‐like properties, yield extracellular vesicles (PEVs) with high affinity for NPCs and enable targeted phenotypic regulation.
Jing Yan, Yazhou Lin, Shuo Miao, Minghao Chao, Chengbai Dai, Ziwen Hao, Rui Chen, Ming Guan, Bin Pan, Fenglei Gao, Quan Zhou   +10 more
wiley   +1 more source

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Simultaneous electroporation and dielectrophoresis in non-electrolytic micro/nano-electroporation [PDF]

, 2018
It was recently shown that electrolysis may play a substantial detrimental role in microfluidic electroporation. To overcome this problem, we have developed a non-electrolytic micro/nano electroporation (NEME) electrode surface, in which the metal ...
Matthew Powell-Palm, Rubinsky, Boris, Powell-Palm, Matthew, Chenang Lyu, Lyu, Chenang, Boris Rubinsky, Wang, Jianping, Jianping Wang   +7 more
core   +1 more source

TH/TRs–COL11A2 Axis Mediates Loss of a Differentiated Astrocyte State in Hypogyrified Brains

Advanced Science, EarlyView.
Using a gyrencephalic congenital hypothyroidism pig model, this study reveals cerebral atrophy and cortical hypogyrification. Single‐cell sequencing identifies astrocytes as major TH‐responsive cells, with the COL11A2‐enriched Astro‐2 state nearly absent.
Ying Zhang, Xiao Wang, Hongyan Ma, Pengcheng Lyu, Shuangjie Tian, Ruojun Zong, Jiani Chen, Gang Ren, Yongjiang Yang, Zichen Yang, Guosong Qin, Naishi Li, Yanfang Wang, Jianwei Jiao, Jianguo Zhao   +14 more
wiley   +1 more source

Layer‐Specific Dermal and Subcutaneous Delivery via Modular LNP–Hydrogel‐Integrated Threaded Microneedles

Advanced Science, EarlyView.
Guided by spatial transcriptomics and clinical validation, a threaded microneedle platform enables precise layer‐specific delivery across the dermis and subcutaneous tissue. By addressing distinct pathogenic remodeling in different skin layers, this strategy coordinates compartment‐specific intervention, restores tissue architecture, and offers a ...
Fangzhou Xie, Yixiang Zhang, Shifeng Ling, Yuxuan Huang, Yingying Wei, Rao Fu, Jiahao He, Baikun Liu, Lucia Zhou, Kaifeng Huo, Yida Wang, Qingfeng Li, Yawei Du, Wenguo Cui, Yun Xie   +14 more
wiley   +1 more source

Calcium electroporation induces tumor eradication, long-lasting immunity and cytokine responses in the CT26 colon cancer mouse model

OncoImmunology, 2017
Electroporation is used in cancer treatment because of its ability to increase local cytotoxicity of e.g. bleomycin (electrochemotherapy) and calcium (calcium electroporation).
Hanne Falk, Patrick F. Forde, Marie Lund Bay, Uma Maheswari Mangalanathan, Pernille Hojman, Declan M. Soden, Julie Gehl   +6 more
doaj   +1 more source

In Vivo Electroporation of Neurons

Cold Spring Harbor Protocols, 2007
INTRODUCTIONThis protocol describes the electroporation of DNA constructs to drive in vivo gene expression in neurons during early chick development. Electroporation is a method of physically introducing DNA constructs into cells through the application of an electric field.
openaire   +3 more sources

Expanding Genetic Code to Generate Human Brain Organoids with Both Vasculature and Microglia‐Like Cells

Advanced Science, EarlyView.
Using genetic code expansion, we engineered vascularized human cerebral organoids (vhCOs) with microglia‐like cells and blood‐brain barrier features. vhCOs recapitulate neurovascular interactions, regional identities, and neuronal subtypes resembling the fetal brain.
Haishuang Lin, Yaqing Wang, Hu Du, Yuxin Qin, Haoyu Zhang, Peng Wang, Lin Wei, Jianhua Qin   +7 more
wiley   +1 more source

Modification of a Chlamydomonas reinhardtii CRISPR/Cas9 transformation protocol for use with widely available electroporation equipment

MethodsX, 2020
A recently reported protocol demonstrates efficient CRISPR/Cas9 gene editing of Chlamydomonas reinhardtii [1]. The published protocol demonstrates transformation and editing of a wall-less strain of C.
Rudolph V. Park, Holly Asbury, Stephen M. Miller   +2 more
doaj   +1 more source