Results 61 to 70 of about 133,618 (201)

High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation

, 2014
We established a high-efficiency nuclear transformation method for the diatom Phaeodactylum tricornutum using an electroporation system. Based on a universal electroporation protocol, the conditions for the introduction of exogenous DNA including ...
Zhang, Chunye, Hu, Hanhua
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A detailed description of an economical setup for electroporation of chick embryos in ovo

Brazilian Journal of Medical and Biological Research, 2013
One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation.
R.M. Borges, J.H. Horne, A. Melo, J.T. Vidal, F.M. Vieceli, M.O. Melo, T.Y.N. Kanno, S.E. Fraser, C.Y.I. Yan   +8 more
doaj   +1 more source

Broad-Host-Range Shuttle Vectors for Screening of Regulated Promoter Activity in Viridans Group Streptococci: Isolation of a pH-Regulated Promoter [PDF]

, 2000
Viridans group streptococci are major constituents of the normal human oral flora and are also identified as the predominant pathogenic bacteria in native valve infective endocarditis.
Zaat, S. A., Kok, Jan, Zaat, SAJ, Kok, J, Dankert, J., Vriesema, A. J., Vriesema, Aldwin J.M.,, Sebastian A. J. Zaat, Brinkman, R., Jan Kok, Jacob Dankert, Zaat, Sebastian A.J.,, Rene ́ Brinkman, Brinkman, René,, Kok, Jan,, Vriesema, AJM, Dankert, Jacob,, Brinkman, René, Vriesema, Aldwin J.M., Aldwin J. M. Vriesema, Zaat, Sebastian A.J.   +20 more
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Conductivity in Jurkat cell suspension after ultrashort electric pulsing

, 2004
Ultrashort electric pulses applied to similar cell lines such as Jurkat and HL-60 cells can produce markedly different results , which have been documented extensively over the last few years.
Kolb, J, Joshi, R P, Garner, A L, Beebe, S, Chen, N, Swanson, R J, Chen, G, Schoenbach, K H   +7 more
core   +1 more source

Dictyostelium discoideum transformation by oscillating electric field electroporation

BioTechniques, 2003
Dictyostelium discoideum has been used as a genetically tractable model organism to study many biological phenomena. High-efficiency transformation is a prerequisite for successful genetic screens such as mutant complementation, identification of ...
Laeticia Alibaud, Pierre Cosson, Mohammed Benghezal   +2 more
doaj   +1 more source

Irreversible electroporation in the treatment of locally advanced pancreas and liver metastases of colorectal carcinoma [PDF]

, 2016
Aim of the study : Irreversible electroporation is a new, non-thermal ablation technique in the treatment of parenchymal organ tumors which uses short high voltage pulses of electricity in order to induce apoptosis of targeted cells.
Dawid Murawa, Jacek Kocur, Mateusz Wichtowski, Piotr Nowaczyk   +3 more
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Recent advances in plant electroporation.

, 1987
The topic is reviewed under the headings (1) induced membrane events; (2) parameters for electroporation and DNA uptake; (3) types of electroporation pulses; and (4) pore parameters, DNA uptake and integration.
Tempelaar, M. J., Tempelaar, M.J., Jones, M.G.K., Jones, H., Jones, M. G. K.   +4 more
core  

Spin-Coating-Based Fabrication of Nanostraw Arrays for Cellular Nano-electroporation

Nano-electroporation technology allows external biomolecules to cross the cell membrane and enter cells. Nanostraws (NSs) with hollow channels are promising nanostructures to provide nano-electroporation for intracellular delivery, yet the preparation ...
Aihua Zhang (174376), Tian Hang (2280229), Meiwan Chen (565048), Xi Xie (310180), Gen He (3237855), Hui-jiuan Chen (12025387), Chuanjie Yao (12025384)   +6 more
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Simultaneous electroporation and dielectrophoresis in non-electrolytic micro/nano-electroporation [PDF]

, 2018
It was recently shown that electrolysis may play a substantial detrimental role in microfluidic electroporation. To overcome this problem, we have developed a non-electrolytic micro/nano electroporation (NEME) electrode surface, in which the metal ...
Matthew Powell-Palm, Rubinsky, Boris, Powell-Palm, Matthew, Chenang Lyu, Lyu, Chenang, Boris Rubinsky, Wang, Jianping, Jianping Wang   +7 more
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An Explorative Study on Calcium Electroporation for Low-risk Basal Cell Carcinoma

Acta Dermato-Venereologica
In electrochemotherapy, permeabilization of the cell membrane by electric pulses increases the anti-tumour effect of chemotherapeutics. In calcium electroporation, chemotherapy is replaced by calcium chloride with obvious benefits.
Stine R. Wiegell, Kristoffer Hendel, Christine S.K. Fuchs, Julie Gehl, Mille Vissing, Sara W. Bro, Jesper T. Troelsen, Gregor B.E. Jemec, Merete Haedersdal   +8 more
doaj   +1 more source