Results 91 to 100 of about 127,262 (303)

Cochlear Implant Close-Field Electroporation

, 2016
Applications for gene therapy that depend upon localization and control of gene expression are challenged by the mode for gene delivery. Viral vectors inherently suffer spread of the viral particles, as does liposome-based gene transfer.
Crawford, Edward N., Housley, GD ; https://orcid.org/, Lovell, N ; https://orcid.org/, Gary D. Housley, Pinyon, Jeremy L., Edward N. Crawford, Matthias Klugmann, Browne, Cherylea J. (R18123), Crawford, EN ; https://orcid.org/, Klugmann, Matthias, Jeremy L. Pinyon, Cherylea J. Browne, Housley, Gary D., Nigel H. Lovell, Klugmann, M ; https://orcid.org/, Pinyon, J ; https://orcid.org/, Lovell, Nigel H., Browne, CJ ; https://orcid.org/   +17 more
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Gasdermin D‐Mediated Release of IL‐33 Results in Fetal Brain Developmental Abnormalities During Maternal Colitis

Advanced Science, EarlyView.
Under colitis, Gsdmd mediates the release of IL‐33 from the epithelium of pregnant mice. IL‐33 can cross the placenta and enhance the proliferative capacity of neural stem cells, ultimately resulting in behavioral deficits in the offspring. Excessive pyroptosis in the colonic epithelium also triggers the translocation of LPS, which in turn increases ...
Huiyang Jia, Shukui Zhang, Kai Ma, Jie Zhou, Jianwei Jiao   +4 more
wiley   +1 more source

Recent advances in plant electroporation.

, 1987
The topic is reviewed under the headings (1) induced membrane events; (2) parameters for electroporation and DNA uptake; (3) types of electroporation pulses; and (4) pore parameters, DNA uptake and integration.
Tempelaar, M. J., Tempelaar, M.J., Jones, M.G.K., Jones, H., Jones, M. G. K.   +4 more
core  

Divergent Roles of mGlu2 and mGlu3 Receptors in Amyloid‐β Production and Cognitive Dysfunctions in Alzheimer's Disease

Advanced Science, EarlyView.
This study explores the opposing effects of the mGluR2 and mGluR3 receptors on amyloid precursor protein processing. mGluR2 promotes amyloidogenic cleavage, while mGluR3 favors non‐amyloidogenic pathways. Using a brain‐penetrant nanobody as a mGluR2 positive allosteric modulator, the study uncovers how its chronic activation aggravates amyloid‐β burden
Pierre‐André Lafon, Mireille Elodie Tsitokana, Ugo Guy Alenda, Yen‐Ling Lian, Clémentine Eva Philibert, Mathieu Oosterlaken, Marta Cimadevila, Gaëlle Dudon, Jessica Monnic, Salomé Roux, Julie Bessié, Séverine Diem, Franck Vandermoere, Laurent Prézeau, Patrick Chames, Julie Kniazeff, Sylvie Claeysen, Anaïs Menny, Jean‐Philippe Pin, Véronique Perrier, Jianfeng Liu, Philippe Rondard   +21 more
wiley   +1 more source

Development of an Efficient Electroporation Method for Iturin A-Producing Bacillus subtilis ZK

, 2015
In order to efficiently introduce DNA into B. subtilis ZK, which produces iturin A at a high level, we optimized seven electroporation conditions and explored an efficient electroporation method.
Shu, Dan, Zhang, Zhi, Ding, Zhong-Tao, Tan, Hong, Luo, Di, Tan, H (reprint author), Chinese Acad Sci, Chengdu Inst Biol, Key Lab Environm & Appl Microbiol, 9 Sect 4,Renmin Nan Rd, Chengdu 610041, Peoples R China.   +5 more
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Endogenous Engineering Reprograms Extracellular Vesicles for Enhanced Therapeutic Function

Advanced Science, EarlyView.
This review explains how Extracellular vesicles‐producing cells can be endogenously engineered to load therapeutic proteins and nucleic acids. We summarize physiological and genetic strategies that harness native sorting pathways for selective cargo loading.
Jinghui Wang, Wenxuan Zhao, Xiaoming Hu, Yishu Wang, Peidong Yang, Diwei Zheng, Yugang Wang, Guanghui Ma, Min Shi, Wei Wei, Shuang Wang   +10 more
wiley   +1 more source

Monitoring of current density and electric field distribution during electroporation of heterogeneous tissues using MR techniques

, 2022
Electrical properties of biological tissues have been of interest for over a century as they determine the pathways of current flow through the body, and are therefore important in the analysis of a wide range of biomedical applications.
Damijan Miklavčič, Marko Strucic, Samo Mahnič-Kalamiza, Igor Serša, Rok Šmerc, Matej Kranjc, Vitalij Novickij, Jessica Genovese   +7 more
core   +1 more source

Electroporation of cells.

, 1990
By measuring uptake of the membrane impermeable dye. phenosafranine, it can be shown that the plasma membrane of intact cells within cell aggregates can be reversibly permeabilized by electroporation.
Jones, M.G.K., Jones, M. G. K., Lindsey, K.   +2 more
core  

tBid‐Mediated Genetic Ablation of Connective Tissue Cells Reveals Their Key Regulatory Function During Limb Regeneration in Axolotls

Advanced Science, EarlyView.
We establish a tBid‐mediated cell ablation system in axolotls, achieve rapid and efficient ablation of multiple cell types, including muscle stem cell, spinal cord cell, and connective tissue (CT) cells. We investigate the role of CT using tBid‐mediated CT ablation and identify its essential role for limb development and regeneration.
Yan Hu, Weimin Feng, Zitian He, Jiayi Zeng, Jingyi Tang, Shulin Li, Tongbo Yu, Sulei Fu, Hui Ma, Binbin Lu, Yanmei Liu, Ji‐Feng Fei   +11 more
wiley   +1 more source

In vivo electroporation-mediated gene delivery to the beating heart [PDF]

, 2014
Gene therapy may represent a promising alternative strategy for cardiac muscle regeneration. In vivo electroporation, a physical method of gene transfer, has recently evolved as an efficient method for gene transfer.
Tevaearai, Hendrik, Giraud, Marie-Noelle, Amiq Gazdhar, Hendrik T. Tevaearai, Martin Flück, Gazdhar, Amiq, Gazdhar, Amiq Ur Rahman, Flück, Martin, Marie-Noëlle Giraud, Tevaearai, Hendrik T   +9 more
core   +1 more source