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Electroporation of Mycobacteria
2015High-efficiency transformation of DNA is integral to the study of mycobacteria, allowing genetic manipulation. Electroporation is the most widely used method for introducing DNA into mycobacterial strains. Many parameters contribute to high-efficiency transformation; these include the species per strain, the transforming DNA, the selectable marker, the
Goude, Renan +2 more
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Micro-/nanoscale electroporation
Lab on a Chip, 2016In this review, the technical aspects of micro- and nanoscale electroporation with their representative cutting-edge research, including gene editing (CRISPR-Cas9), adoptive immunotherapy, and cellular reprogramming. The challenges and opportunities of advanced electroporation technologies are discussed.
Lingqian, Chang +6 more
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Analytical and Bioanalytical Chemistry, 2010
Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell.
Manyan, Wang +3 more
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Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell.
Manyan, Wang +3 more
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Electroporation of Mycobacteria
2003High-efficiency transformation is a major limitation in the study of mycobacteria. The genus Mycobacterium can be difficult to transform; this is mainly caused by the thick and waxy cell wall but is compounded by the fact that most molecular techniques have been developed for distantly related species such as Escherichia coli and Bacillus subtilis.
T, Parish, N G, Stoker
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2009
Membrane electroporation (MEP) uses short high-voltage pulses to render cell membranes transiently porous and therewith permeable to otherwise impermeable substances. This technique was first described, in vitro, by Neumann in 1982. In vivo, this method is restricted to solid tissues accessible to the electrodes used to apply the electric field pulses.
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Membrane electroporation (MEP) uses short high-voltage pulses to render cell membranes transiently porous and therewith permeable to otherwise impermeable substances. This technique was first described, in vitro, by Neumann in 1982. In vivo, this method is restricted to solid tissues accessible to the electrodes used to apply the electric field pulses.
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