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Culture Media and Embryo Culture
, 2021 Before considering the basis of embryo culture medium, it is worthwhile reflecting on its function. A detailed overview of embryo metabolism is given in Chapter 4; however, in brief, the embryo must satisfy changing demands for energy by consumption of ...
Sturmey, Roger G. +3 more
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Whole embryo culture of presomitic mouse embryos
Toxicology in Vitro, 1995 Rat embryos explanted at the presomite stage and cultured through limb bud stages develop to well formed embryos and exhibit growth and differentiation which mimic those observed in vivo at corresponding stages.
Gofflot, Françoise +3 more
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Bioscientifica Proceedings, 2020 Pig embryos can be cultured using a number of different strategies including complex approaches like culture in vivo in a surrogate oviduct (rabbit, sheep, mouse), culture in mouse oviducts in organ culture, and co-culture of embryos with cells in addition to simple approaches like culture in defined media or salt solutions. Addition of serum to medium
R M, Petters, K D, Wells
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The Effect of Light on Embryos and Embryo Culture [PDF]
Journal of Reproductive and Stem Cell Biotechnology, 2012 In oviparous animals, the egg hatches outside of the body and is exposed to light; in some cases throughout the development of the fetus. In mammals, fertilization and the growth of embryos in vivo occurs in the dark but in human IVF, these embryos are exposed to variable light sources and intensities.
Kimball O. Pomeroy, Michael Reed
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Pluripotency in the Embryo and in Culture [PDF]
Cold Spring Harbor Perspectives in Biology, 2012 Specific cells within the early mammalian embryo have the capacity to generate all somatic lineages plus the germline. This property of pluripotency is confined to the epiblast, a transient tissue that persists for only a few days. In vitro, however, pluripotency can be maintained indefinitely through derivation of stem cell lines.
Jennifer, Nichols, Austin, Smith
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Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System [PDF]
International Journal of Fertility and Sterility, 2008 Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes.
Sayyed Morteza Hosseini +11 more
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F&S Reports, 2021 How embryos are cultured, in groups or individually, can influence their development and have other unforeseen impacts on subsequent assisted reproductive technologies. Although a group culture of embryos improves the blastocyst formation rates, this can
Jason E. Swain, Ph.D.
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Pushing the Limits: Day 7 Embryo Culture
Fertility & Reproduction, 2022 Improvements in embryo culture systems, based on the dynamic metabolic requirements of the embryo have facilitated the extension of embryo culture to the blastocyst stage.
Lisa Y. S. LEE
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Can Culture Media Impact Preimplantation Embryo Aneuploidy?
Fertility & Reproduction, 2021 With continued improvements in blastocyst culture, cell sampling approaches, and genetic analysis platforms, the resulting improvements in embryo development and the resolution and accuracy of chromosome analysis have provided valuable insights into the ...
Jason E. Swain
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Digital Microfluidic Dynamic Culture of Mammalian Embryos on an Electrowetting on Dielectric (EWOD) Chip. [PDF]
PLoS ONE, 2015 Current human fertilization in vitro (IVF) bypasses the female oviduct and manually inseminates, fertilizes and cultivates embryos in a static microdrop containing appropriate chemical compounds.
Hong-Yuan Huang +7 more
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