CellsClassical preimplantation embryo culture is performed in static fluid environments. Whether a dynamic fluid environment, like the fallopian tube, is beneficial for embryo development remains to be determined across mammalian species.
Jose Roberto Alegretti +8 more
doaj +1 more source
Embryo culture in presence of oviductal fluid induces DNA methylation changes in bovine blastocysts [PDF]
, 2017 During the transit through the oviduct, the early embryo initiates an extensive DNA methylation reprogramming of its genome. Given that these epigenetic modifications are susceptible to environmental factors, components present in the oviductal milieu ...
Barrera, Antonio Daniel +7 more
core +1 more source
Chorioallantoic membrane assay as model for angiogenesis in tissue engineering : focus on stem cells [PDF]
, 2020 Tissue engineering aims to structurally and functionally regenerate damaged tissues, which requires the formation of new blood vessels that supply oxygen and nutrients by the process of angiogenesis.
Bronckaers, Annelies +6 more
core +3 more sources
In Vitro Culture of Mammalian Embryos: Is There Room for Improvement?
CellsPreimplantation embryo culture, pivotal in assisted reproductive technology (ART), has lagged in innovation compared to embryo selection advancements. This review examines the persisting gap between in vivo and in vitro embryo development, emphasizing ...
Roberto Gualtieri +6 more
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Embryo culture - Methods and protocols
European Journal of Histochemistry, 2013 It has been a really pleasure to follow the presentation of all the topics related to the improvements occurring in human embryo culture as they are here presented.
Carlo Alberto Redi
doaj +1 more source
Human embryos from overweight and obese women display phenotypic and metabolic abnormalities [PDF]
, 2014 STUDY QUESTION Is the developmental timing and metabolic regulation disrupted in embryos from overweight or obese women? SUMMARY ANSWER Oocytes from overweight or obese women are smaller than those from women of healthy weight, yet post-fertilization ...
Leary, C. +5 more
core +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Review: Role of tubal environment in preimplantation embryogenesis: application to co-culture assays [PDF]
, 2017 The culture of early preimplantation stage embryo is still delicate and the metabolic pathways of embryos are not completely understood. Embryo needs are evolutionary during the preimplantation development, consequently it is difficult to meet embryo ...
Guérin, Pierre, Ménézo, Yves
core
Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development [PDF]
, 2012 BACKGROUNDS: Previous studies suggested that endocrine disruptors (ED) are toxic on preimplantation embryos and inhibit development of embryos in vitro culture.
Hae-Hyeog Lee +3 more
core +1 more source