Results 131 to 140 of about 299,455 (330)

Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]

, 2009
Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core   +1 more source

Identification and characterization of a new HNH restriction endonuclease with unusual properties [PDF]

, 2023
Meghna Santoshi, Bharat Engleng, Sachin M. Eligar, Immadi Siva Ratnakar, Easa Nagamalleshwari, Valakunja Nagaraja   +5 more
openalex   +1 more source

Adaptive Machine Learning Framework for Optimizing the Affinity Purification of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viral (AAV) vectors for gene therapy are becoming integral to modern medicine, providing therapeutic options for diseases once deemed incurable. Currently, viral vector purification is a critical bottleneck in the gene therapy industry, impacting product efficacy and safety as well as accessibility and cost to patients ...
Kelvin P. Idanwekhai, Shriarjun Shastry, Morgan R. Hurst, Arianna Minzoni, Eduardo Barbieri, Luke Remmler, Eugene N. Muratov, Michael A. Daniele, Stefano Menegatti, Alexander Tropsha   +9 more
wiley   +1 more source

APE1 Activity is Controlled by Non‐G‐Quadruplex Conformations in Single‐ and Double‐Stranded G‐Quadruplex Constructs

Chemistry – A European Journal, EarlyView.
G‐quadruplex (G4) folding in DNA is inhibitory to AP endonuclease, although the protein still binds well to G4s. Curiously, partially folded G‐rich sequences, possibly transient hairpins, containing AP are very good substrates for cleavage by APE1. Herein, the enzyme activity and G4 folding are studied in single‐stranded vs. duplex contexts.
Brianna L. Trabucco, Aaron M. Fleming, Cynthia J. Burrows   +2 more
wiley   +1 more source

549 VISUALIZATION OF HUMAN GLOBIN GENES BY RESTRICTION ENDONUCLEASE MAPPING: A NEW APPROACH TO THE PRENATAL DIAGNOSIS OF GLOBIN GENE DELETION [PDF]

, 1978
Stuart H. Orkin, David G. Nathan
openalex   +1 more source

Highly Potent Fluorogenic Ligands for Triplex DNA: 5‐Substituted 2‐(Naphthalen‐2‐yl)‐4H‐Chromen‐4‐Ones

Chemistry – A European Journal, EarlyView.
5‐Substituted 2‐(naphthalen‐2‐yl)‐4H‐chromen‐4‐ones are reported as a novel class of highly potent and selective triplex DNA ligands. These ligands induce triplex formation at submicromolar concentrations and inhibit enzymatic activity via ligand‐mediated triplex formation.
Nghia Tran, Landy Gu, Krege M. Christison, Kalee Thao, Liang Xue   +4 more
wiley   +1 more source

Molecular Docking and Identification of Candidate Blockers for Endonuclease Domain of Lassa Virus Polymerase as Potential Drugs

, 2022
J.B. Minari, E.E. Agho, F.D. Adebiyi, O.O. Rotimi, Balikis Olabisi SHOLAJA, J.O. Adejumo   +5 more
openalex   +2 more sources

Population Pharmacokinetic and Exposure‐Efficacy Analysis of Baloxavir Marboxil for Influenza Treatment and Post‐Exposure Prophylaxis in Children

Clinical Pharmacology &Therapeutics, EarlyView.
Baloxavir acid (baloxavir), the active metabolite of the prodrug baloxavir marboxil, is a selective inhibitor of the influenza virus cap‐dependent endonuclease. The population pharmacokinetic (popPK) profile of baloxavir in adults/adolescents has been described previously.
Sylvie Retout, Stefan De Buck, Jacques Gaudreault, Sébastien Jolivet, Vincent Duval, Valérie Cosson, Marie‐Laure Delporte   +6 more
wiley   +1 more source

Association betweenapurinic/apyrimidinic endonuclease 1rs1760944 T>G polymorphism and susceptibility of cancer: a meta-analysis involving 21764 subjects [PDF]

, 2019
Guowen Ding, Yu Chen, Huiwen Pan, Hao Qiu, Weifeng Tang, Shuchen Chen   +5 more
openalex   +1 more source

Commonalities of platelet dysfunction in heart failure with preserved ejection fraction and underlying comorbidities

ESC Heart Failure, Volume 12, Issue 2, Page 1013-1028, April 2025.
Abstract Heart failure with preserved ejection fraction (HFpEF) is characterized by a lack of a specific targeted treatment and a complex, partially unexplored pathophysiology. Common comorbidities associated with HFpEF are hypertension, atrial fibrillation, obesity and diabetes.
Giorgia D'Italia, Blanche Schroen, Judith M.E.M. Cosemans   +2 more
wiley   +1 more source