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Endonucleases

Plant Molecular Biology, 2000
Programmed cell death (PCD) involves hydrolysis of genomic DNA, which must be catalyzed by endonuclease(s) capable of digesting dsDNA. Plants have two major classes of endonucleases active towards dsDNA, Zn2+-dependent endonuclease and Ca2+-dependent endonuclease.
M, Sugiyama   +3 more
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Endonucleases

Current Protocols in Molecular Biology, 1989
AbstractReaction conditions for numerous ensonucleases are detailed in this unit along with discussions of potential applications. Specific enzymes include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease and DNase I.
S, Tabor, K, Struhl
openaire   +2 more sources

Restriction Endonuclease

CRC Critical Reviews in Biochemistry, 1976
This review provides a comprehensive account of the current status of the biology and biochemistry of restriction endonucleases. Both Class I and Class II restriction endonucleases will be considered. However, emphasis will be placed on the Class II group, which recognizes and cleaves a specific duplex DNA sequence.
Richard J. Roberts, Kenneth Murray
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Endonucleases

Current Protocols in Molecular Biology, 2011
AbstractReaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonucleases to generate nonspecific breaks in dsDNA is also ...
openaire   +2 more sources

The endonuclease IV family of apurinic/apyrimidinic endonucleases

Mutation Research/Reviews in Mutation Research, 2010
Apurinic/apyrimidinic (AP) endonucleases are versatile DNA repair enzymes that possess a variety of nucleolytic activities, including endonuclease activity at AP sites, 3' phosphodiesterase activity that can remove a variety of ligation-blocking lesions from the 3' end of DNA, endonuclease activity on oxidative DNA lesions, and 3' to 5' exonuclease ...
James M, Daley   +2 more
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Restriction Endonucleases

Angewandte Chemie International Edition in English, 1978
AbstractRestriction endonucleases are enzymes commonly found in bacteria which can fragment DNA. Cleavage of the DNA molecule takes place after recognition of a nucleotide sequence which is specific for each restriction endonuclease. Some of the enzymes split the DNA within the recognition sequence. This property allows these enzymes to be used for the
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Endonucleases

Biochimica et biophysica acta-Molecular basis of disease, 2014
Mouse transgenesis has been instrumental in determining the function of genes in the pathophysiology of human diseases and modification of genes by homologous recombination in mouse embryonic stem cells remains a widely used technology. However, this approach harbors a number of disadvantages, as it is time-consuming and quite laborious.
Wijshake, Tobias   +2 more
openaire   +1 more source

Control of structure-specific endonucleases to maintain genome stability

Nature reviews. Molecular cell biology, 2017
P. Dehé, P. Gaillard
semanticscholar   +1 more source

Genome-wide analysis reveals specificities of Cpf1 endonucleases in human cells

Nature Biotechnology, 2016
Daesik Kim   +5 more
semanticscholar   +1 more source

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