Results 151 to 159 of about 6,098 (159)
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The Cmr complex: an RNA-guided endoribonuclease
Biochemical Society Transactions, 2013The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) system protects prokaryotes from infection by viruses and other potential genome invaders. This system represents an inheritable and adaptable immune system that is mediated by large ribonucleoprotein complexes, the CRISPR–Cas effector complexes.
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Designing Efficient and Specific Endoribonuclease-Prepared siRNAs
2012RNA interference (RNAi) has grown to be one of the main techniques for loss-of-function studies, leading to the elucidation of biological function of genes in various cellular systems and model organisms. While for many invertebrates such as Drosophila melanogaster (D. melanogaster) and Caenorhabditis elegans (C.
Surendranath V +3 more
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Structure and function of Zucchini endoribonuclease in piRNA biogenesis
Nature, 2012PIWI-interacting RNAs (piRNAs) silence transposons to maintain genome integrity in animal germ lines. piRNAs are classified as primary and secondary piRNAs, depending on their biogenesis machinery. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters in the genome through the primary processing pathway ...
Miharu K. Kamatani +16 more
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Endoribonuclease from bovine adrenal cortex cytosol
Biochemistry, 1975An endoribonuclease which digests a variety of synthetic homoribopolymers and poly(A)-rich mRNA has been identified and purified greater than 500-fold with respect to specific activity from bovine adrenal cortex cytosol. Enzymatic digestion of synthetic poly(riboadenylic acid) was stimulated by Mn-2+ and Mg-2+ and the enzyme exhibited broad pH and salt
Henry I. Miller +2 more
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Endoribonuclease IV. 2. Further Investigation on the Specificity
European Journal of Biochemistry, 1976The poly(A)-specific endoribonuclease IV produces oligo(A) fragments of a chain length of 10 AMP nucleotides. One enzyme molecule performs 1700 cleavages per min; the cleavages occur randomly. The endoribonuclease IV is a nuclear enzyme which is present in the oviduct of quails in a concentration of 40 000 enzyme molecules per cell. Poly(A) segments on
Gerhard Seibert +3 more
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Cleavage site recognition by the tRNA splicing endoribonuclease
Gene, 1993A single tRNA-splicing endoribonuclease can cleave several precursors. In addition to the conserved nucleotides (nt), there are positions in the mature domain that, though not always occupied by the same nt, nevertheless play a fundamental role in intron excision reaction. The elements of the recognition set (invariant nt, nt at the cardinal positions)
M. Irene Baldi +3 more
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Endoribonuclease activity associated with animal RNA viruses
Journal of Virology, 1978A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis of Sendai virus was incubated with the precursor to Escherichia coli tRNA Tyr. The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU.
S Altman, Daniel Kolakofsky
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Endoribonucleases – enzymes gaining spotlight in mRNA metabolism
The FEBS Journal, 2010The efficient turnover of messenger RNA represents an important mechanism that allows the cell to control gene expression. Until recently, the mechanism of mRNA decay was mainly attributed to exonucleases, comprising enzymes that degrade RNAs from the ends of the molecules.
Chow H. Lee, Wai Ming Li, Tavish Barnes
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Ire1p: A Kinase and Site-Specific Endoribonuclease
2003Tania N. Gonzalez, Peter Walter
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