Results 101 to 110 of about 399,723 (294)

Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy

Advanced Materials, EarlyView.
Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa, Gracia Mendoza, Silvia Irusta, Manuel Arruebo   +3 more
wiley   +1 more source

Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells [PDF]

, 2015
BACKGROUND: Engineered nanoparticles (NP) are being developed for inhaled drug delivery. This route is non-invasive and the major target; alveolar epithelium provides a large surface area for drug administration and absorption, without first pass ...
Ruenraroengsak, P, Tetley, TD
core   +2 more sources

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, EarlyView.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]

, 2016
Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen, Braeckmans, Kevin, Remaut, Katrien   +2 more
core   +1 more source

Understanding and Overcoming Antibody‐Drug Conjugate Resistance: Biological Mechanisms and Emerging Analytical Frameworks in Breast Cancer

Advanced Science, EarlyView.
Antibody–drug conjugates (ADCs) transform breast cancer therapy, yet resistance limits their durability. Emerging evidence reveals that ADC failure is not solely tumor‐intrinsic but shaped by dynamic tumor–microenvironment interactions that alter drug delivery, processing, and response.
Minji Seo, Jangsoon Lee, Naoto T. Ueno
wiley   +1 more source

Enhancing MicroRNA Activity through Increased Endosomal Release Mediated by Nigericin

Molecular Therapy: Nucleic Acids, 2019
The therapeutic promise of small-RNA therapeutics is limited, not only by the lack of delivery vehicles, but also by the inability of the small RNAs to reach intracellular compartments where they can be biologically active.
Esteban A. Orellana, Ahmed M. Abdelaal, Loganathan Rangasamy, Srinivasarao Tenneti, Sunghyun Myoung, Philip S. Low, Andrea L. Kasinski   +6 more
doaj   +1 more source

163. Hepatocyte Targeted Endosomal Escape Agents [PDF]

Molecular Therapy, 2015
The successful development of potent non-viral DNA and mRNA delivery agents to transfect liver hepatocytes in vivo requires the use of an endosomal escape molecule. Two major strategies have emerged, one that utilizes membrane lytic peptides, and a second that uses proton sponge polymers. Both approaches would require administration of excess endosomal
White, Christopher W., Baumhover, Nicholas J., Rice, Kevin G.   +2 more
openaire   +1 more source

Visualization of Endosomal Escape of Intracellularly Delivered Protein With Unexpected Photochemical Internalization of Trypan Blue

Advanced Science, EarlyView.
TB, a conventional cell viability dye, acts as both a fluorescence modulator (quenching GFP fluorescence upon protein binding and recovering it upon release) and a photosensitizer that triggers endosomal escape via photochemical internalization (PCI) under 590 nm light.
Zhongqi Yao, Zhan Shi, Peng Hu, Changping Wang, Hui Wang   +4 more
wiley   +1 more source

Localized NF‐κB Inhibition Reduces Lipid Nanoparticle‐Associated Inflammation

Advanced Science, EarlyView.
Lipid nanoparticles (LNPs) activate NF‐κB and provoke inflammation that limits therapeutic delivery. By encapsulating selective NF‐κB inhibitors within LNPs, localized suppression of inflammatory signaling is achieved without compromising mRNA expression.
Carolann L. Espy, Jichuan Wu, Serena Omo‐Lamai, Liuqian Wang, Fengyi Dong, Aleksa Milosavljevic, Sarah O'Neill, Taylor Brysgel, Yufei Wang, Michael Zaleski, Emily Wolfe, Rhea Maheshwari, Manthan Patel, Dirk Trauner, Jia Nong, Zhicheng Wang, Jacob S. Brenner   +16 more
wiley   +1 more source

Single-cell quantification and dose-response of cytosolic siRNA delivery

Nature Communications, 2023
Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during ...
Hampus Hedlund, Hampus Du Rietz, Johanna M. Johansson, Hanna C. Eriksson, Wahed Zedan, Linfeng Huang, Jonas Wallin, Anders Wittrup   +7 more
doaj   +1 more source