Results 141 to 150 of about 399,723 (294)

A Solution for Exosome‐Based Analysis: Surface‐Enhanced Raman Spectroscopy and Artificial Intelligence

Advanced Intelligent Discovery, EarlyView.
Exosomes are emerging as powerful biomarkers for disease diagnosis and monitoring. This review highlights the integration of surface‐enhanced Raman spectroscopy with artificial intelligence to enhance molecular fingerprinting of exosomes. Machine learning and deep learning techniques improve spectral interpretation, enabling accurate classification of ...
Munevver Akdeniz, Zakarya Al‐Shaebi, Omer Aydin   +2 more
wiley   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Engineered internal architecture of core-shell lipid nanoparticles promotes efficient mRNA endosomal release

Nature Communications
Messenger RNA (mRNA) therapeutics rely on lipid nanoparticles (LNPs) for delivery, yet inefficient endosomal escape remains a major bottleneck, with only a small fraction of internalized cargo reaching the cytoplasm. Conventional LNPs encapsulate mRNA in
Tianyao Li, Jingxin Zhang, Jing Guo, Bohong Sun, Yuxuan Han, Hu Xu, Yi Weng, Qinghao Cao, Min Li, Guangyu Zhao, Lin Liu, Xingfa Gao, Lianpan Dai, Daming Wang, Yuhong Cao   +14 more
doaj   +1 more source

Cellular delivery of antibodies: effective targeted subcellular imaging and new therapeutic tool [PDF]

, 2010
It is already more than a century since the pioneering work of the Nobel Laureate Ehrlich gave birth to the side chain theory1, which helped to define antibodies and their ability to target specific biological sites.
Andrew L. Lewis, Giuseppe Battaglia, Irene Canton, Marzia Massignani, Nicholas J. Warren, Nisa Patikarnmonthon, Steven P. Armes   +6 more
core   +1 more source

Antibody translocation into the cytosol: Mechanisms and engineering of endosomal escape

Journal of Controlled Release
The development of cytosol-penetrating immunoglobulin G (IgG) antibodies that autonomously enter target cells and reach the cytosol is challenging despite their potential to enable the therapeutic targeting of intracellular antigens and delivery of functional payloads.
Dae-Seong, Kim, Yong-Sung, Kim
openaire   +2 more sources

Comparison of Receptor‐Mediated Endocytosis and Its Application to Enhance DNA Transfection by TFAMoplex

Advanced NanoBiomed Research, EarlyView.
We developed an assay to distinguish cellular binding from internalization. Compatible with microscopy and high‐throughput screening, the method identifies ligand‐mediated uptake. Applying top candidates to a protein‐based DNA carrier enhanced transfection efficiency, providing a rational strategy to improve non‐viral gene delivery systems.
David Scherer, Steffen Honrath, Jonas Kurmann, Alexander Klipp, Finn Brigger, Jean‐Christophe Leroux   +5 more
wiley   +1 more source

Polyfluoroalkyl‐Tagged Cell‐Penetrating Peptide‐Additives Enhance Intracellular Protein Delivery via Sustained Monomeric Lipid Interaction

Angewandte Chemie International Edition, EarlyView.
We introduce cell‐penetrating peptides (CPPs) with polyfluoroalkyl‐tags that drastically enhance intracellular protein delivery by promoting membrane retention. Via the combination of live‐cell imaging, biophysical analysis, and computational simulations, we reveal how fluorous CPPs interact with membranes via monomeric interactions and dynamic tag ...
Sarah Hansen, Hana Zupan, Ferhat Mutlu, Jan Vincent V. Arafiles, Rubén Cruz, Palina Dubatouka, Kenichi Ataka, Martin Lehmann, Bettina G. Keller, Joachim Heberle, Christian P. R. Hackenberger   +10 more
wiley   +1 more source

Escaping to silence using an endosome-disrupting polymer

Molecular Therapy, 2021
Jamie L, Betker, Scott G, Tilden, Thomas J, Anchordoquy   +2 more
openaire   +2 more sources

A Novel Amino Lipid Series for mRNA Delivery: Improved Endosomal Escape and Sustained Pharmacology and Safety in Non-human Primates.

Molecular Therapy, 2018
The success of mRNA-based therapies depends on the availability of a safe and efficient delivery vehicle. Lipid nanoparticles have been identified as a viable option.
S. Sabnis, E. Kumarasinghe, Timothy Salerno, C. Mihai, T. Ketova, Joe J. Senn, A. Lynn, A. Bulychev, I. McFadyen, Joyce Chan, Ö. Almarsson, M. Stanton, K. Benenato   +12 more
semanticscholar   +1 more source

PEG Alternatives in Liposomal Nanocarriers: Physicochemical Characteristics and Sustainable Process Strategies

Asia-Pacific Journal of Chemical Engineering, EarlyView.
ABSTRACT The global trend toward sustainable and intensified bioprocesses is driving innovation in the design and scalable synthesis of liposomal nanocarriers, a cornerstone of modern drug delivery. For decades, these nanosystems have relied exclusively on polyethylene glycol (PEG) for their sustained circulation in vivo, but they are currently ...
Chandra Has
wiley   +1 more source