Results 131 to 140 of about 7,363,445 (247)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The Effect of Precursor Concentration on the Particle Size, Crystal Size, and Optical Energy Gap of Ce_xSn_1-xO₂ Nanofabrication. [PDF]

Nanomaterials (Basel), 2021
Al-Hada NM, Md Kasmani R, Kasim H, Al-Ghaili AM, Saleh MA, Banoqitah EM, Alhawsawi AM, Baqer AA, Liu J, Xu S, Li Q, Noorazlan AM, Ahmed AAA, Flaifel MH, Paiman S, Nazrin N, Ali Al-Asbahi B, Wang J.   +17 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Séminaire GAP 'Savoirs et Idéologies en Politique': JC Croizet

, 2013
Savoirs et idéologies en politique Genèse, circulation, transmission Séminaire du Groupe d'Analyse Politique Université Paris Ouest Nanterre — Plan du Campus Bâtiment F – Salle des Conférences (352) - 14h-16h30 Chères/Chers collègues, pour notre ...
gap
core   +1 more source

Driving the Emission Towards Blue by Controlling the HOMO-LUMO Energy Gap in BF₂ -Functionalized 2-(Imidazo[1,5-a]pyridin-3-yl)phenols. [PDF]

Chemistry, 2021
Colombo G, Attilio Ardizzoia G, Furrer J, Therrien B, Brenna S.   +4 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Defying the inverse energy gap law: a vacuum-evaporable Fe(ii) low-spin complex with a long-lived LIESST state. [PDF]

Chem Sci, 2023
Grunwald J, Torres J, Buchholz A, Näther C, Kämmerer L, Gruber M, Rohlf S, Thakur S, Wende H, Plass W, Kuch W, Tuczek F.   +11 more
europepmc   +1 more source