Results 111 to 120 of about 9,256,798 (287)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Reduce wear and control friction for copper-based powder metallurgy wet friction material by optimizing porosity, surface roughness and surface texture

Alexandria Engineering Journal
Copper-based powder metallurgy materials are common friction materials in many fields such as aviation, high-speed railway and automobiles because of their good thermal conductivity, stable friction performance and relatively high mechanical properties ...
Zhi Chen, Yuedu Chen, Wuzhong Tang, Zhizhong Zhang, Weihai Xue, Huiming Guo, Fenglin Han   +6 more
doaj   +1 more source

Folder 8: Model engine data sheets and operating instructions, O continued, 1986, 1988, 2005, 2010

, 1986
Instructions for O.S. FS-60M four stroke cycle marine model engine.
O.S. Engine Manufacturing Company, Ltd.
core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Metallurgy-inspired multi-modal design and interfacial strengthening of heterogeneous lattice structures for superior compressive performance

Materials & Design
Inspired by the synergistic strengthening mechanisms of heterogeneous grain structures in metallurgy, this study proposes a novel multi-modal design strategy that integrates global architectural layout design with local interfacial strengthening.
Beiouyang Chen, Xiangyu Li, Yibo Li, Minghui Huang, Sheng Ding   +4 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Mechanism of heterogeneous substrate stimulating growth for nickel-based single crystal superalloy

Journal of Materials Research and Technology
Minimizing the deviation of single-crystal (SX) primary orientation can significantly enhance the yield rate, service life, and performance of turbine blades or castings.
Binqiang Wang, Senhong Chen, Long Zeng, Mingxu Xia, Jianguo Li, Kun Bu   +5 more
doaj   +1 more source

Model engine data sheets and operating instructions, E - F, ca. 1950, 1991-1993, 1997, 1999

, 1950
Operating instructions and parts list for the Feeney 4-Cycle model ...
Feeny Engine Company
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source