Results 41 to 50 of about 8,833,176 (232)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Identification of eccentricity for disc milling cutter of indexable two sided inserts
Advances in Mechanical Engineering, 2021 Tool eccentricity has a significant impact on machining quality, accuracy, and operation status of machine tool. It is difficult to accurately identify tool eccentricity.
Gensheng Li, Chao Xian, Hongmin Xin
doaj +1 more source
FEBS Letters, EarlyView.Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source
Journal of Materials Research and TechnologyThis study investigates the influence of abrasive tool wear on the surface topography and microstructure evolution of GH4169G during polishing, combining experiments and simulations to assess surface functionality.
Rui Yang +3 more
doaj +1 more source
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
FEBS Letters, EarlyView.GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
Nihon Kikai Gakkai ronbunshu, 2015 The purpose of this study is formulating the reduced integration method of damage elasto plastic constitutive model with divided back stress based on full implicit framework.
Shogo SANNOMARU +2 more
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Current status and prospects of aviation remanufacturing technology based on cold spray
Hangkong gongcheng jinzhanCold spray additive manufacturing technology，with its unique "solid-state deposition" process characteristics，has shown great application potential in the repair and remanufacturing of damaged aerospace structures.
CHI Yada +4 more
doaj +1 more source