Results 111 to 120 of about 369,296 (265)

A penalty-based algorithm proposal for engineering optimization problems. [PDF]

Neural Comput Appl, 2023
Oztas GZ, Erdem S.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Optimizing for the Artificial Intelligence Driven Search Era: An Integrated Framework for Search Engine Optimization, Generative Engine Optimization, and Answer Engine Optimization

INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH IN ENGINEERING AND MANAGEMENT
Abstract - The evolution of search technologies from traditional keyword-based retrieval toward generative and answer-based paradigms has fundamentally altered digital visibility strategies. Conventional Search Engine Optimization (SEO), while still essential, is increasingly insufficient for ensuring content presence in environments dominated by ...
Gembali Manasa, Dr. P. T. Raju, Suravaram Sai Ravindra Reddy, Shaik Riyaz, Syed Nausheen   +4 more
openaire   +1 more source

Mother optimization algorithm: a new human-based metaheuristic approach for solving engineering optimization. [PDF]

Sci Rep, 2023
Matoušová I, Trojovský P, Dehghani M, Trojovská E, Kostra J.   +4 more
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Energy valley optimizer: a novel metaheuristic algorithm for global and engineering optimization. [PDF]

Sci Rep, 2023
Azizi M, Aickelin U, A Khorshidi H, Baghalzadeh Shishehgarkhaneh M.   +3 more
europepmc   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

Reptile Search Algorithm Considering Different Flight Heights to Solve Engineering Optimization Design Problems. [PDF]

Biomimetics (Basel), 2023
Yao L, Li G, Yuan P, Yang J, Tian D, Zhang T.   +5 more
europepmc   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source