Scientific Reports, 2020 Subterranean ecosystems are understudied and challenging to conventionally survey given the inaccessibility of underground voids and networks. In this study, we conducted a eukaryotic environmental DNA (eDNA) metabarcoding survey across the karst ...
Katrina M. West +5 more
doaj +1 more source
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species [PDF]
Royal Society Open Science, 2018 Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine ...
Kim M. Parsons +3 more
doaj +1 more source
Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment [PDF]
, 2014 peer-reviewedRecent advances made in “omics” technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in ...
Couldrey, Christine, Doherty, Rachael
core +2 more sources
MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species [PDF]
Royal Society Open Science, 2015 We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
M. Miya +11 more
doaj +1 more source
Environmental variables, habitat discontinuity and life history shaping the genetic structure of Pomatoschistus marmoratus [PDF]
, 2014 Coastal lagoons are semi-isolated ecosystems exposed to wide fluctuations of environmental conditions and showing habitat fragmentation. These features may play an important role in separating species into different populations, even at small spatial
A Pérez-Ruzafa +119 more
core +3 more sources
Environmental DNA illuminates the dark diversity of sharks [PDF]
Science Advances, 2018 Environmental DNA reveals unsuspected shark diversity and calls for monitoring and protection of residual populations.
Michel Kulbicki +17 more
openaire +11 more sources
Scientific Reports, 2021 Associations between the human gut microbiome and health outcomes continues to be of great interest, although fecal sample collection methods which impact microbiome studies are sometimes neglected. Here, we expand on previous work in sample optimization,
Jacquelyn Jones +4 more
doaj +1 more source
Screening Universal COⅠ Primers for eDNA Metabarcoding of Freshwater Fishes on Hainan Island
Progress in Fishery Sciences, 2023 The known universal primers for environmental DNA (eDNA) metabarcoding of fish are mainly located in the mitochondrial ribosome gene regions. Not only are the reference sequences insufficient but also some relative fish species could not be identified ...
Zhi CHEN +8 more
doaj +1 more source
An MLSA-based online scheme for the rapid identification of Stenotrophomonas isolates [PDF]
, 2011 An online scheme to assign Stenotrophomonas isolates to genomic groups was developed using the multilocus sequence analysis (MLSA), which is based on the DNA sequencing of selected fragments of the housekeeping genes ATP synthase alpha subunit (atpA ...
Barbosa, Heloiza Ramos +8 more
core +3 more sources
Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis [PDF]
, 2011 Background\ud DNA methylation is an epigenetic mechanism associated with regulation of gene expression and it is modulated during chemical carcinogenesis.
Chipman, J Kevin +4 more
core +4 more sources