Results 21 to 30 of about 1,506,434 (336)

Metagenomics: DNA sequencing of environmental samples [PDF]

Nature Reviews Genetics, 2005
Although genomics has classically focused on pure, easy-to-obtain samples, such as microbes that grow readily in culture or large animals and plants, these organisms represent only a fraction of the living or once-living organisms of interest. Many species are difficult to study in isolation because they fail to grow in laboratory culture, depend on ...
Tringe, Susannah Green, Rubin, Edward M.
openaire   +3 more sources

Environmental variables, habitat discontinuity and life history shaping the genetic structure of Pomatoschistus marmoratus [PDF]

, 2014
Coastal lagoons are semi-isolated ecosystems exposed to wide fluctuations of environmental conditions and showing habitat fragmentation. These features may play an important role in separating species into different populations, even at small spatial
A Pérez-Ruzafa, A Pérez-Ruzafa, AN Yoboué, BL Fluker, BM Fitzpatrick, BS Weir, BS Weir, C Lemaire, C Mazzoldi, C Oosterhout van, C Pampoulie, C Parisod, C Riginos, C Riginos, C Vergara-Chen, C Vergara-Chen, C Vergara-Chen, Carlos Vergara-Chen, CC Keever, CH Pashley, CL Richards, D Chessel, D Hedgecock, D Hedgecock, D Paetkau, D Rungis, D Verdiell Cubedo, DA Earl, DE Ruzzante, DG Roberts, DJ Marshall, DO Conover, DS Portnoy, DT Bilton, E Boissin, E Daemen, E Sanford, EE Nielsen, ES Gysels, F Giménez-Casalduero, F Maltagliati, F Pascalis De, F Rousset, Freitas R QuintinoV, G Cognetti, G Evanno, G González-Wangüemert, G González-Wangüemert, GH Pogson, IR Bradbury, J Marques, J Martinez-Garrido, J Sambrook, JB Horne, JC Avise, JD Durand, JD Hogan, JF Marques, JK Pritchard, JM Cano, JM Cornuet, K Lindström, K Tarnowska, L Camilli, L Excoffier, L Excoffier, L Hauser, L Wang, LB Beheregaray, LC Larsson, LL Cavalli-Sforza, M Fonds, M García-Sánchez, M Giovannotti, M González-Wangüemert, M González-Wangüemert, M González-Wangüemert, M González-Wangüemert, M González-Wangüemert, M González-Wangüemert, M Raymond, M Trabelsi, Mercedes González-Wangüemert, MF Canino, MHD Larmuseau, MHD Larmuseau, MHD Larmuseau, MHD Larmuseau, ML Johansson, MP Chapuis, MR Christie, O Bardin, P Beerli, P Berrebi, P Berrebi, P Berrebi, PJ Miller, PM Bisol, R Mejri, R Mejri, R Mejri, RJ Watts, RS Waples, S Consuegra, S Hirase, S Innocentiis De, S Kraitsek, S Planes, S Wood, S Wood, S Wright, SN Wood, T Antao, T Hastie, T Snäll, V Milana, WN Venables, WR Rice, WT McCraney, Á Pérez-Ruzafa   +119 more
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Making environmental DNA count [PDF]

Molecular Ecology Resources, 2015
The arc of reception for a new technology or method – like the reception of new information itself – can pass through predictable stages, with audiences’ responses evolving from ‘I don't believe it’, through ‘well, maybe’ to ‘yes, everyone knows that’ to, finally, ‘old news’.
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MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species [PDF]

Royal Society Open Science, 2015
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding environmental DNA (eDNA) from fishes. Primers were designed using aligned whole mitochondrial genome (mitogenome) sequences from 880 species, supplemented by partial mitogenome ...
M. Miya, Y. Sato, T. Fukunaga, T. Sado, J. Y. Poulsen, K. Sato, T. Minamoto, S. Yamamoto, H. Yamanaka, H. Araki, M. Kondoh, W. Iwasaki   +11 more
doaj   +1 more source

Evaluating detection limits of next-generation sequencing for the surveillance and monitoring of international marine pests [PDF]

, 2013
Most surveillance programmes for marine invasive species (MIS) require considerable taxonomic expertise, are laborious, and are unable to identify species at larval or juvenile stages.
Bott, Nathan J., Pochon, Xavier, Smith, Kirsty Fiona, Wood, Susanna A.   +3 more
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DNA repair systems and the pathogenesis of Mycobacterium tuberculosis: varying activities at different stages of infection [PDF]

, 2010
Mycobacteria, including most of all MTB (Mycobacterium tuberculosis), cause pathogenic infections in humans and, during the infectious process, are exposed to a range of environmental insults, including the host's immune response.
Adams, Afanasev, Alina E. Gorna, Aly, Arnold, Bacon, Banu, Bergval, Betts, Boshoff, Boshoff, Bostanabad, Burney, Capelli, Chan, Chan, Cheng, Cooper, Cubillos-Ruiz, Darwin, Darwin, Das, Davis, Dos Vultos, Dubnau, Durbach, Farnia, Fennelly, Filliol, Fleischmann, Flint, Fontan, Friedberg, Frischkorn, Fu, Fu, Gaynor, Gordhan, Graham, Grishko, Guthlein, Hampshire, Hazbon, Hughes, Jain, Jaroslaw Dziadek, Jiao, Kapur, Karakousis, Karakousis, Karunkaran, Katti, Kesavan, Kim, Ko, Ko, Korycka-Machala, Krokan, Kurthkoti, Kurthkoti, Lari, Lever, Loudon, MacMicking, Malshetty, Martinez, Mokrousov, Moller, Movahedzadeh, Mulder, Munoz-Elias, Muttucumaru, Nguyen, Niemann, Nikolayevskyy, Nouvel, O'sullivan, Olano, Peccia, Peccia, Pitcher, Rachman, Rachman, Rad, Ramaswamy, Richard P. Bowater, Riley, Saito, Sander, Sassetti, Sassetti, Saunders, Schnappinger, Schwab, Sherman, Sinha, Sinha, Smeulders, Springer, Srivastava, Stephanou, Talaat, Talaat, Tang, Tsai, Venkatesh, Vilcheze, Voskuil, Voskuil, Waddell, Wanner, Warner, Weikert, Wiid, Wiseman, World Health Organization, Zaczek, Zainuddin   +117 more
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The California environmental DNA “CALeDNA” program [PDF]

, 2019
AbstractGlobal change is leading to habitat shifts that threaten species persistence throughout California’s unique ecosystems. Baseline biodiversity data provide opportunities for ecosystems to be managed for community complexity and connectivity.
Meyer, Rachel, Curd, Emily E, Schweizer, Teia, Gold, Zack, Ruiz Ramos, Dannise, Shirazi, Sabrina, Kandlikar, Gaurav, Kwan, Wai-Yin, Lin, Meixi, Friese, Amanda, Moberg-Parker, Jordan, Munguia Ramos, Miroslava, Shapiro, Beth, Sexton, Jason, Pipes, Lenore, Garcia Vedrenne, Ana, Palacios Mejia, Maura, Aronson, Emma, Moore, Tiara, Nielsen, Rasmus, Lewin, Harris, Barber, Paul, Wall, Jeff, Kraft, Nathan, Wayne, Robert   +24 more
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Estimating species richness using environmental DNA [PDF]

Ecology and Evolution, 2016
AbstractThe foundation for any ecological study and for the effective management of biodiversity in natural systems requires knowing what species are present in an ecosystem. We assessed fish communities in a stream using two methods, depletion‐based electrofishing and environmental DNA metabarcoding (eDNA) from water samples, to test the hypothesis ...
Olds, Brett P., Jerde, Christopher L., Renshaw, Mark A., Li, Yiyuan, Evans, Nathan T., Turner, Cameron R., Deiner, Kristy, Mahon, Andrew R., Brueseke, Michael A., Shirey, Patrick D., Pfrender, Michael E., Lodge, David M., Lamberti, Gary A.   +12 more
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Fecal sample collection methods and time of day impact microbiome composition and short chain fatty acid concentrations

Scientific Reports, 2021
Associations between the human gut microbiome and health outcomes continues to be of great interest, although fecal sample collection methods which impact microbiome studies are sometimes neglected. Here, we expand on previous work in sample optimization,
Jacquelyn Jones, Stacey N Reinke, Alishum Ali, Debra J Palmer, Claus T. Christophersen   +4 more
doaj   +1 more source

Screening Universal COⅠ Primers for eDNA Metabarcoding of Freshwater Fishes on Hainan Island

Progress in Fishery Sciences, 2023
The known universal primers for environmental DNA (eDNA) metabarcoding of fish are mainly located in the mitochondrial ribosome gene regions. Not only are the reference sequences insufficient but also some relative fish species could not be identified ...
Zhi CHEN, Xingwei CAI, Zhixin SHEN, Qingfeng ZHANG, Fangyuan LI, Yuan GU, Gaojun LI, Guangjun ZHAO, Zhenjiang WANG   +8 more
doaj   +1 more source