Results 21 to 30 of about 6,899,226 (260)

Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays [PDF]

, 2009
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van, Gent-Pelzer, M.P.E., van, Van Doorn, R., Doorn, R. van, Speksnijder, A., Speksnijder, A.G.C.L., Klerks, M.M., Schoen, C.D., van Gent-Pelzer, M., Klerks, M., Gent-Pelzer van, M., Doorn, R., van, Kowalchuk, G.A., van Gent-Pelzer, M.P.E.   +13 more
core   +2 more sources

Phylogenetic classification of short environmental DNA fragments [PDF]

, 2008
Krause L, Diaz NN, Goesmann A, et al. Phylogenetic classification of short environmental DNA fragments. Nucleic Acids Research. 2008;36(7):2230-2239.Metagenomics is providing striking insights into the ecology of microbial communities.
Nattkemper, Tim Wilhelm ; https://orcid.org/, Krause, Lutz, Kelley, Scott, Stoye, Jens, Stoye, Jens ; https://orcid.org/, Diaz, Naryttza N., Goesmann, Alexander ; https://orcid.org/, Nattkemper, Tim W., Rohwer, Forest, Goesmann, Alexander, Edwards, Robert A.   +10 more
core   +1 more source

Revising the range of Rocky Mountain tailed frog, Ascaphus montanus, in British Columbia, Canada, using environmental DNA methods [PDF]

, 2020
The Rocky Mountain tailed frog, Ascaphus montanus , is a species at‐risk in Canada. Based upon time‐ and area‐constrained physical search surveys completed between 1996 and 2004, its Canadian distribution was defined as occurring in 19 tributaries and ...
Round, Jessica M., Bergman, Lauren C., Allison, Michael J., Helbing, Caren C., Adams, Ian T., Allen, Heather, Mirabzadeh, Ali, Goldberg, Caren S., Hobbs, Jared   +8 more
core   +1 more source

Water, water everywhere: environmental DNA can unlock population structure in elusive marine species [PDF]

Royal Society Open Science, 2018
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine ...
Kim M. Parsons, Meredith Everett, Marilyn Dahlheim, Linda Park   +3 more
doaj   +1 more source

Droplet digital PCR applied to environmental DNA, a promising method to estimate fish population abundance from humic-rich aquatic ecosystems [Elektronisk resurs]

, 2021
Measures of environmental DNA (eDNA) concentrations in water samples have the potential to be both a cost-efficient and a nondestructive method to estimate fish population abundance.
Capo, Eric,, Sveriges lantbruksuniversitet., Königsson, Helena,, Spong, Göran,   +3 more
core   +2 more sources

Reporting the limits of detection and quantification for environmental DNA assays [PDF]

, 2019
Background Environmental DNA (eDNA) analysis is increasingly being used to detect the presence and relative abundance of rare species, especially invasive or imperiled aquatic species.
Mangan, Anna M., Merkes, Christopher M., Piaggio, Antoinette J., Richter, Catherine A., Allison, Michael J., Klymus, Katy E., Stokdyk, Joel P., Wilson, Chris C., Helbing, Caren C., Jackson, Craig A., Hunter, Margaret E., Monroe, Emy M., Goldberg, Caren S., Lance, Richard F.   +13 more
core   +1 more source

Screening Universal COⅠ Primers for eDNA Metabarcoding of Freshwater Fishes on Hainan Island

Progress in Fishery Sciences, 2023
The known universal primers for environmental DNA (eDNA) metabarcoding of fish are mainly located in the mitochondrial ribosome gene regions. Not only are the reference sequences insufficient but also some relative fish species could not be identified ...
Zhi CHEN, Xingwei CAI, Zhixin SHEN, Qingfeng ZHANG, Fangyuan LI, Yuan GU, Gaojun LI, Guangjun ZHAO, Zhenjiang WANG   +8 more
doaj   +1 more source

Discovery of cyanophage genomes which contain mitochondrial DNA polymerase [PDF]

, 2011
DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that
Whitworth, Anna L., Holmes, Antony B., Mohr, Remus, Hess, Wolfgang R., Nicholas H. Mann, David J. Scanlan, Remus Mohr, Scanlan, David J., Antony B. Holmes, Anthony W. Larkum, Martha R. J. Clokie, Clokie, Martha R. J., Anna L. Whitworth, Yi-Wah Chan, Millard, Andrew D., Wolfgang R. Hess, Mann, Nicholas H., Andrew D. Millard, Chan, Yi-Wah, Larkum, Anthony W. D.   +19 more
core   +1 more source

Fecal sample collection methods and time of day impact microbiome composition and short chain fatty acid concentrations

Scientific Reports, 2021
Associations between the human gut microbiome and health outcomes continues to be of great interest, although fecal sample collection methods which impact microbiome studies are sometimes neglected. Here, we expand on previous work in sample optimization,
Jacquelyn Jones, Stacey N Reinke, Alishum Ali, Debra J Palmer, Claus T. Christophersen   +4 more
doaj   +1 more source

The complete mitochondrial genome of the Mekong Giant Salmon Carp (Aaptosyax grypus): a tool to conserve an emblematic and elusive megafish

Knowledge and Management of Aquatic Ecosystems
The giant salmon carp is a Critically Endangered megafish from the Mekong River. Despite its emblematic species status, it is rarely observed, and less than 30 confirmed specimens have been recorded since the original species description in 1991.
Condachou Céline, Hogan Zeb S., Chan Bunyeth, Chhut Chheana, Heng Kong, Chhuoy Samol, Brosse Sébastien   +6 more
doaj   +1 more source