Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
The Environmental Consequence of Early Colorectal Cancer Detection: A Literature Review of the Environmental Impact Assessment of Colorectal Cancer Diagnostic Pathways. [PDF]
Osinkolu I, Lacey A, Harris D.
europepmc +1 more source
Techno-economic analysis and environmental impact assessment of biodiesel production from bio-oil derived from microwave-assisted pyrolysis of pine sawdust. [PDF]
Makepa DC +3 more
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Environmental impact assessment of leachate from mining tailings using electrical resistivity imaging. [PDF]
Ali MAH +6 more
europepmc +1 more source
Environmental impact assessment of transportation and land alteration using Earth observational datasets: Comparative study between cities in Asia and Europe. [PDF]
Mhana KH +3 more
europepmc +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
A quantitative environmental impact assessment of Australian ultra-processed beverages and impact reduction scenarios - ERRATUM. [PDF]
Anastasiou K +6 more
europepmc +1 more source
Innovative Bioplasticizers from Residual Cynara cardunculus L. Biomass-Derived Levulinic Acid and Their Environmental Impact Assessment by LCA Methodology. [PDF]
Ruini C +9 more
europepmc +1 more source
Assessing farm sustainability with value oriented methods [PDF]
Agricultural policy makers aim to combine strong economic performance with a sustainable use of natural resources. An important step is to move from trying to define sustainability towards developing concrete tools for measuring and promoting ...
Van Passel, Steven
core +1 more source

