Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Progress in the study of product methodology development and competitiveness assessment model of voluntary carbon offsets. [PDF]
Han J, Zheng S, Yuan J, Zhang C.
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
The impacts of development finance for climate on public health outcomes and life satisfaction: evidence from developing countries in the global south. [PDF]
Borojo DG, Liu J.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Findings From the Pearl River Basin: Dissolved Oxygen Dominates Functional Trait Filtering and Diversity Patterns in Urban Rivers. [PDF]
Huang J +9 more
europepmc +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
Multiscale environmental analysis on autotrophic euglenid communities: insights from DNA metabarcoding. [PDF]
Jankowska K +3 more
europepmc +1 more source
From global political agreement to public health practice: operationalising the Belém adaptation indicators for health. [PDF]
Palmeiro-Silva Y +5 more
europepmc +1 more source
Public health quality indicators as a prioritization and leadership tool: a scoping review of their role in health system transformation. [PDF]
Triantafyllou C +5 more
europepmc +1 more source

