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Enzymatically active colloidal crystal arrays

Journal of Colloid and Interface Science, 2009
We report the construction of three-dimensional (3D) colloidal crystal arrays (CCA) and hollow colloidal crystal arrays (HCCA) derived from the self-assembly of polyelectrolytes (PE)-coated polystyrene (PS) particles and their use as models of high surface area systems to immobilize peroxidase (POD).
Yu, A., Liang, Z.
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Enzymatic Activity of Human Carboxylesterases

Current Protocols in Toxicology, 2007
AbstractThe carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane‐enriched fractions of the liver. This unit describes assays used to measure the enzymatic activity and tissue distribution of human CEs ...
Matthew K, Ross, Abdolsamad, Borazjani
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Microwave Activation of Enzymatic Catalysis

Journal of the American Chemical Society, 2008
Microwave irradiation can be used to regulate biocatalysis. Herein, the utilization of hyperthermophilic enzymes in a microwave reactor is reported. While these enzymes are inactive at low temperatures, they can be activated with microwave irradiation.
Douglas D, Young   +3 more
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Enzymatic activity in the activated-sludge floc matrix

Applied Microbiology and Biotechnology, 1995
The enzymatic activity of activated sludge was investigated with special emphasis on the localization of the enzymes in the sludge floc matrix. Activated sludge from an advanced activated-sludge treatment plant, performing biological N and P removal, was used. An enzymatic fingerprint was established using a panel of six different enzymes.
Frølund, B., Griebe, T., Nielsen, P. H.
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Enzymatic activity of aphroproteins.

Biochemistry. Biokhimiia, 1999
Chitinase and proteinase activities were found in aphroproteins excreted by larvae of the cicada Aphrophora costalis Mats; this accounts for their fungicidal effect. Aphroproteins did not show DNase or RNase activities and did not exhibit properties of proteinase inhibitors.
Maksyutova N.   +5 more
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Enzymatic activity in single cells

2019
With the increasing recognition of the importance in addressing cell-to-cell variations for the understanding of complex biological systems, single cell analyses are becoming increasingly important. Presented in this chapter is a highly sensitive approach capable of measuring human topoisomerase 1 (TOP1) activity in single CD133 positive DLD-1 cells ...
Keller, Josephine Geertsen   +2 more
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Non-enzymatic and enzymatic activation of mitomycin C: Identification of a unique cytosolic activity

International Journal of Cancer, 1996
Mitomycin C (MMC), an alkylating anti-tumor agent, was activated by non-enzymatic and enzymatic mechanisms leading to DNA binding and adduct formation. However, it was enzymatically, not non-enzymatically, activated MMC which induced inter-strand DNA cross-linking, a major determinant of cell death.
P, Joseph, Y, Xu, A K, Jaiswal
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Fluorescence Activated Cell Sorting for Enzymatic Activity

Combinatorial Chemistry & High Throughput Screening, 2006
Directed evolution is a reliable method for protein engineering and as a tool for investigating structure/function relationships. A key for a successful directed evolution experiment is oftentimes the screen. Fluorescence activated cell sorting (FACS) is powerful high-throughput screening approach to isolate and identify mutants from large protein ...
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Enzymatic activity under pressure

MRS Bulletin, 2017
Abstract
Claus Czeslik   +2 more
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Enzymatic activity of histones

Science, 2020
Histone Function Eukaryotic histones serve as structural elements to package DNA. However, they contain a copper-binding site for which the biological relevance is unknown. Copper homeostasis is critical for several fundamental eukaryotic processes, including mitochondrial respiration. Attar et al. hypothesized that histones may play a critical role in
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