Results 171 to 180 of about 6,595,197 (312)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Structural and (Pseudo-)Enzymatic Properties of Neuroglobin: Its Possible Role in Neuroprotection. [PDF]

Cells, 2021
De Simone G, Sbardella D, Oddone F, Pesce A, Coletta M, Ascenzi P.   +5 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity. [PDF]

Int J Mol Sci, 2021
Grahl MVC, Uberti AF, Broll V, Bacaicoa-Caruso P, Meirelles EF, Carlini CR.   +5 more
europepmc   +1 more source

Lipase enzymatic microreactor in polysaccharide hydrogel: structure and properties

, 2019
© 2019, Springer Science+Business Media, Inc. An enzymatic microreactor was designed on the basis of sodium alginate hydrogel containing a lipolytic enzyme (Candida rugosa lipase) in the polymer matrix pores.
Zuev Y., Rogov A., Bogdanova L., Zueva O.   +3 more
core  

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Enzymatic properties, pathogenic potential, and prevalence of ExfA, a novel Staphylococcus felis exfoliative toxin. [PDF]

J Vet Med Sci
Guo X, Ragab RF, Elfadadny A, Iyori K, Toyoda Y, Nishifuji K.   +5 more
europepmc   +1 more source

Minor Impact of A258D Mutation on Biochemical and Enzymatic Properties of Leishmania infantum GDP-Mannose Pyrophosphorylase. [PDF]

Microorganisms, 2022
Mao W, Lazar N, van Tilbeurgh H, Loiseau PM, Pomel S.   +4 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Screening of mutants, enzymatic properties, and scale-up fermentation for catalysis of naringin using recombinant <i>α</i>-L-rhamnosidase. [PDF]

Front Microbiol
Wang C, Liang L, Ni B, Chen W, Zhang Y, Li H.   +5 more
europepmc   +1 more source