Results 121 to 130 of about 394,097 (307)

Gut Microbiota Regulates Systemic Inflammatory Response and Compensatory Anti‐Inflammatory Response Syndromes by Targeting PF4+ Macrophages in Acute Pancreatitis

Advanced Science, EarlyView.
ABSTRACT Acute pancreatitis (AP) begins with pancreatic local inflammation, leading to the onset of systemic inflammatory response syndrome (SIRS), followed by compensatory anti‐inflammatory response syndrome (CARS), which causes immune paralysis and higher mortality rate.
Liwei Liu, Guanqun Li, Dongxu Lu, Haoran Ding, Tianqi Lu, Yuhang Sui, Can Zhang, Yu Xie, Rui Kong, Hua Chen, Xuewei Bai, Hongtao Tan, Dongbo Xue, Xianzhi Meng, Le Li, Bei Sun   +15 more
wiley   +1 more source

Zika IgM antibody-capture enzyme-linked immunosorbent assay

This document describes the use of an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for the presumptive detection of antibodies to Zika virus in persons meeting Centers for Disease Control and Prevention (CDC) clinical and/or ...

core  

Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays

, 2011
The aim of this project was to demonstrate and develop a confocal readout method for fluorescent immunosorbent assays and investigate its potential advantages in comparison to traditional immunoassays.
Ghafari, H
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CDK4/6 Inhibition Induces CD8+ T Cell Antitumor Immunity via MIF‐Induced Functional Orchestration of Tumor‐Associated Macrophages

Advanced Science, EarlyView.
CDK4/6 inhibition promotes CD8+ T cell expansion through tumor‐macrophage crosstalk by activating HIF‐1α and enhancing MIF‐CD44/CD74 signaling. This reprograms TAMs to boost MHC‐I antigen presentation, and CDK4/6 inhibitor‐trained M1 TAM supernatant therapy synergizes with low‐dose PD‐1 blockade to restore antitumor immunity.
Lin He, Yuzhong Peng, Lat‐lun Leong, Jingbo Zhou, Dongyang Tang, Weilu Wang, Xiaoran Wu, Josh haipeng Lei, Yongqin Ye, Yangyang Feng, Yunfeng Qiao, Xiangpeng Chu, Di Mu, Qi Zhao, Tzuming Liu, Yan Chen, Paul Kwonghang Tam, Chu‐Xia Deng   +17 more
wiley   +1 more source

A novel assay for analysis of the regulation of the function of human osteoclasts.

, 2006
BACKGROUND: Very little is known of the regulation of the function of human osteoclasts, largely due to the virtual impossibility of obtaining human osteoclasts ex vivo.
Shiroo Masahiro, Samuelsson, B, Kirstein Barrie, Chambers, TJ, Barrie Kirstein, Shiroo, M, Fuller, K, Urszula Grabowska, Karen Fuller, Grabowska Urszula, Masahiro Shiroo, Grabowska, U, Samuelsson Bertil, Chambers Timothy J, Fuller Karen, Bertil Samuelsson, Timothy J Chambers, Kirstein, B   +17 more
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LC3B Mediated SETDB1‐Accounted Alcoholic Steatohepatitis via Lipidation‐Dependent LAP and Lipidation‐Independent Nuclear Stabilization

Advanced Science, EarlyView.
SETDB1 is progressively downregulated in ALD, correlating with disease severity. SETDB1 deficiency impairs LAP by disrupting Rubicon membrane localization, leading to defective lipid droplet clearance. Concurrently, loss of SETDB1 reduces nuclear LC3B, causing R‐loop accumulation and cGAS‐STING‐driven inflammation. Lipidated LC3B mediates LAP‐dependent
Yi Zhang, Tan Wei, Jiahang Wu, Chuixu Lin, Dongbo Zhu, Yanhui Li, Shuting Shi, Shishun Huang, Leiming Jiang, Hongzhi Wang, Meiqi Song, Pengfei Gao, Xu Wu, Mingjian Fan, Chaofeng Wei, Qian Wang, Lihui Qu, Zhigang Wang   +17 more
wiley   +1 more source

A técnica ELISA (Enzyme-linked immunosorbent assay)

, 1982
No presente trabalho faz-se uma descrição da técnica ELISA (Enzyme- -Linked Immunosorbent Assay). Referem-se os vários parâmetros de execução desta técnica, as possibilidades de conservação das amostras e das placas sensibilizadas e de reutilização das ...
Varennes, Amarilis de
core   +2 more sources

Electroacupuncture Improves the Learning and Memory by Modulating Hippocampal Glucose Metabolism through IGF1/IGF1R Signaling in Alzheimer's Disease

Advanced Science, EarlyView.
Electroacupuncture (EA) ameliorates learning and memory function in 5×FAD mice by regulating the brain glucose metabolic network. This neuroprotective effect is closely related to enhancing neuronal energy utilization via the IGF1/IGF1R signaling pathway.
Shengxiang Liang, Qingqing Zhang, Xiuxiu Wang, Lixin Peng, Xinru Ji, Jiawei Wang, Yusi Zhang, Jiayang Huang, Junchao Yang, Shiqi Zhan, Yiping Xiao, Weilin Liu, Lidian Chen   +12 more
wiley   +1 more source

Multiplexed nanoparticle-based immunoassays

, 2010
Multiplexed immunoassays have been explored using the fluorescent and luminescent properties of fluorophores and nanoparticles. Epi-fluorescence microscopy, confocal laser scanning microscopy and programmable array microscopy were used to detect signals ...
Murray, KA
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A 3D In Vitro Model of the Human Hepatobiliary Junction

Advanced Science, EarlyView.
A 3D human organoid platform reconstructs the hepatobiliary junction between primary adult hepatocytes and intrahepatic cholangiocytes. These adult hepatobiliary organoids (aHBOs) support directional bile transport from canaliculi to ductule‐like structures, enable quantitative imaging of junction dynamics, and reveal cell‐type‐specific vulnerabilities
Ashley D. Westerfield, Katarzyna A. Grzelak, Katie Katsuyama, Vardhman Kumar, Bess M. Miller, Joa Yun, Jesse Kirkpatrick, David Mankus, Margaret E. Bisher, Abigail K.R. Lytton‐Jean, Z. Gordon Jiang, David D. Lee, Christopher S. Chen, Sangeeta N. Bhatia   +13 more
wiley   +1 more source