Results 101 to 110 of about 3,900,452 (305)

Enzyme-linked immunosorbent assay of IL-8.

, 2013
The hMSCs and Ca9-22 cells were challenged with a MOI of 1∶100 for 1, 2, 4 and 24 h with the microorganism (A) Fusobacterium nucleatum ATTCC 23726 (B)Fusobacterium nucleatum ATTCC 25586 (C) Porphyromonas gingivalis W50 or (D) Porphyromonas gingivalis W83.
Katja Kriebel (479331), Bernd Kreikemeyer (192821), Anne Biedermann (479332), Hermann Lang (479333)   +3 more
core   +1 more source

Silk Fibroin Nanofiber–Immobilized Horseradish Peroxidase Enables Continuous Preparation of Injectable Hydrogels With Negligible Residual Enzyme for Drug Delivery

Advanced Materials Technologies, EarlyView.
Overcoming the immunogenicity challenges of enzyme‐mediated crosslinking, a flow‐through reactor utilizing SFNF‐HRP enables the continuous production of injectable hydrogels. This continuous platform ensures homogeneous crosslinking while minimizing residual HRP in the resulting hydrogels.
Ayari Miyawaki, Shinji Sakai
wiley   +1 more source

CD9+ B Cells Induce T Follicular Helper Cell Apoptosis to Regulate Germinal Center Regression

Advanced Science, EarlyView.
A unique subset of CD9+ B cells regulates germinal center B (GC‐B) cell regression by inducing T follicular helper (Tfh) cell apoptosis through multiple signaling pathways, such as ALCAM‐CD6. FOXP1 plays a key role in GC‐B cell differentiation. These findings clarify mechanisms of GC degeneration and termination and offer potential targets for adenoid ...
Wenjing Liao, Lijuan Song, Meiqian Xu, Tianhao Liang, Yixuan Chen, Gui Chen, Zaihuan Lin, Huakang Du, Guofei Feng, Zhiming Feng, Qi Zhang, Fan Pan, Saixuan Yang, Xinyin Zhang, Wenxu Peng, Tianlong Li, Zhuoshen Lin, Yabang Chen, Jiayi Liu, Baoxin Peng, Yan Liu, Xing Niu, Ting Li, Chengyu Huang, Ke Mo, Jianlei Xie, Yu Zou, Xiaowen Zhang   +27 more
wiley   +1 more source

Heterogeneous Immunoassay Using Channels and Droplets in a Digital Microfluidic Platform

Micromachines, 2019
This work presents a heterogeneous immunoassay using the integrated functionalities of a channel and droplets in a digital microfluidic (DMF) platform. Droplet functionality in DMF allows for the programmable manipulation of discrete sample and reagent ...
Yuguang Liu, Ian Papautsky
doaj   +1 more source

Quantification of Cibenzoline by Enzyme-Linked Immunosorbent Assay

YAKUGAKU ZASSHI, 2007
We have developed an enzyme-linked immunosorbent assay (ELISA) suitable for routine monitoring of serum levels of cibenzoline. Anti-cibenzoline antibody was obtained by immunizing rabbits with cibenzoline conjugated with bovine serum albumin using N-(4-maleimidobutyryloxy)succinimide as a heterobifunctional coupling agent. An enzyme marker was prepared
SAITA, Tetsuya, TOKUNAGA, Akira, EGOSHI, Masayoshi, TOKUSHIMA, Hideaki, FUJITO, Hiroshi   +4 more
openaire   +3 more sources

Accuracy parameters of immunodiffusion and enzyme-linked immunosorbent assay tests.

, 2015
DID:double agar gel immunodiffusion; ELISA: enzyme-linked immunosorbent assay; 1: antigen the Aspergillus fumigatus; 2: pool de antigens the A. fumigatus, A. flavus e A.
Lídia Raquel de Carvalho (557816), Rinaldo Poncio Mendes (365963), Tatiane Fernanda Sylvestre (553786), Daniela Vanessa Moris (557813), Priscila Zacarias de Azevedo (783296), Ricardo de Souza Cavalcante (553785), Maria Luiza Cotrim Sartor de Oliveira (513131)   +6 more
core   +1 more source

Gonadotropin‐Inhibitory Hormone (GnIH) and Its Receptor GPR147 Mediates Acute Stress‐Induced Activation of the Locus Coeruleus‐SAM Axis

Advanced Science, EarlyView.
ABSTRACT Gonadotropin‐inhibitory hormone (GnIH, also known as RFamide‐related peptide‐3, RFRP‐3) is a hypothalamic neuropeptide that regulates vertebrate reproduction via its receptor GPR147. Although GnIH is known to mediate stress‐induced reproductive suppression, its role in broader stress responses remains unclear.
Jiani Zhang, Hongyu Zhu, Wenqi Wang, Bingqian Shen, Yuanyuan Xin, Xingxing Song, Xuanyi Lu, Lixuan Li, Siting Chen, Xiaochen Zhang, Jialing Zeng, Haodong Zheng, Jianlong Lao, Ke Peng, Yixian Wei, Jiaming Zheng, Chuanhuo Hu, Xiaoye Wang, Xun Li   +18 more
wiley   +1 more source

An Adipocyte MKK3–p38β Axis Preserves Insulin Responsiveness in Mice and Is Reciprocally Altered in Human Obesity

Advanced Science, EarlyView.
The adipocyte MKK3–p38β axis emerges as an adaptive stress‐response pathway during obesity. Human visceral adipose tissue analyses, adipocyte‐specific mouse models, and primary adipocytes show that the MKK3–p38β axis supports insulin responsiveness by restraining p70S6K‐dependent inhibitory IRS1 feedback and preserving AKT–GLUT4 signaling. This pathway
Edgar Bernardo, Nuria Matesanz, Jorge‐Luis Torres, Alba C. Arcones, Juan Ignacio Jiménez‐Loygorri, Alfonso Mora, Leticia Herrera‐Melle, Luis Leiva‐Vega, Maria Elena Rodriguez, Lourdes Hernández‐Cosido, Marta Leon, Paula Yong González, Teresa Durán, Tamara Parracho, Francisca Mulero, Rubén Nogueiras, J. Simon C. Arthur, Angel R. Nebreda, Roger J. Davis, Miguel Marcos, Guadalupe Sabio   +20 more
wiley   +1 more source

Monitoring of Salivary Secretory Immunoglobulin A Quantified Two Methods During High-Altitude Volleyball Training Camp

Physiologia
Background/Objectives: Volleyball training camps are known to reduce salivary secretory immunoglobulin A (s-SIgA); however, when it begins to decrease is unclear.
Ryota Sone, Kenji Yamamoto, Shinsuke Tamai, Honoka Goji, Kenji Ohishi   +4 more
doaj   +1 more source

Microglial Deubiquitinase OTUD7B Stabilizes STAT3 to Promote Neuroinflammation and Cognitive Decline in Alzheimer's Disease

Advanced Science, EarlyView.
. ABSTRACT Neuroinflammation driven by microglial activation is a defining feature of Alzheimer's disease (AD), yet the molecular mechanisms sustaining this proinflammatory state remain unclear. Here, we identify the deubiquitinase OTUD7B as a critical regulator of microglial activation and AD pathology.
Luyao Li, Hao Tang, Qin Yu, Yuyang Zhang, Binglu Shi, Ying Kong, Leiyu Xu, Jingjing Shao, Chenghong Hu, Lingyu She, Zhe Wang, Haiyi Chen, Aleksandr V. Samorodov, Gang Cao, Xia Zhao, Yi Wang   +15 more
wiley   +1 more source