A one-step enzyme-linked immunosorbent assay for fipronil detection
A rapid enzyme-linked immunosorbent assay (ELISA) system for the detection of fipronil was studied. Artificial antigens were synthesized through the glutaraldehyde method, and monoclonal antibodies were obtained via immunization and fusion techniques ...
Yujing Li +6 more
doaj +1 more source
Circulating PCSK9 levels and CETP plasma activity are independently associated in patients with metabolic diseases [PDF]
Additional file 1: Figure S1. Correlations between CETP activity and CETP mass (A) and between PCSK9 and CETP mass (B) in patients with T2DM (n=30).
Daiana Ibarretxe +6 more
core +2 more sources
The ELISA, Enzyme-Linked Immunosorbent Assay [PDF]
Featured Article: Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871–4.1 This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University.
openaire +2 more sources
CX3CL1 in Early Detection of Alzheimer's Disease: Plasma Dynamics Across Age and Disease Stages
ABSTRACT Backgrounds Alzheimer's disease (AD) is characterized by amyloid‐beta plaques, tau tangles, and neuroinflammation. C‐X3‐C motif chemokine ligand 1 (CX3CL1, also known as fractalkine), a neuroimmune chemokine implicated in AD pathogenesis, shows inconsistent alterations in plasma/serum across studies.
Ling Wang +6 more
wiley +1 more source
False-positive fourth-generation HIV test associated with autoimmune hemolytic anemia. Case report
Introduction: The fourth-generation ELISA human immunodeficiency virus (HIV) screening test has a high sensitivity and specificity >99% to detect both antigens and antibodies. Estimates are that only 0.5% yield false positive results. Case description:
Santiago Sánchez-Pardo +4 more
doaj +1 more source
ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan +9 more
wiley +1 more source
Gold-black micropillar electrodes for microfluidic ELISA of bone metabolic markers [PDF]
Modification with gold black considerably increased the detection current of protruding micropillar electrodes in microfluidic electrochemical enzyme-linked immunosorbent assay (ELISA)
Fukuda Junji +6 more
core +1 more source
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon +11 more
wiley +1 more source
The place and role of serologic methods in detecting Helicobacter pylori infection [PDF]
The aim of the study was to determine the place and role of serologic methods in detecting Helicobacter pylori (H. pylori) infection, on the basis of estimated enzyme-linked immunosorbent assay (ELISA) and complement fixation test (CFT) sensitivity and ...
Dominis, Mara +4 more
core
Enhanced release of elastase is not concomitant with increased secretion of granulocyte-activating cytokines in whole blood from patients with sepsis [PDF]
Die Diskussion am Ende des Artikels wurde gelöscht (§137l UrhG).Background The proteolytic enzyme elastase released by granulocytes (polymorphonuclear leukocytes [PMN]) in high concentrations during sepsis causes degradation of essential plasma proteins,
Ertel, Wolfgang +6 more
core +1 more source

