Results 61 to 70 of about 395,671 (307)

The enzyme-linked immunosorbent assay (ELISA): history, theory and application

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Enzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents.
Yu. N. Tarakanova, A. D. Dmitriev, D. A. Dmitriev, V. F. Lavrov, Yu. S. Massino, А. A. Pechelyulko, O. L. Segal   +6 more
doaj   +1 more source

A Sandwich Enzyme-linked Immunosorbent Assay for Nattokinase [PDF]

Bioscience, Biotechnology, and Biochemistry, 1994
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for nattokinase, a subtilisin-like fibrinolytic enzyme in the fermented soybean food, natto. The assay method was developed using a combination of a murine anti-nattokinase monoclonal antibody coated on the microtiter well as a catching antibody and rabbit anti-nattokinase
Yuki, Yoshikazu, Nakagawa, Tomoyo, Fujita, Mitsugu, Asada, Aki, Nakanishi, Koichiro, Kato, Kazuo   +5 more
openaire   +1 more source

GAD65 Antibody ELISA With Extended Reportable Range: Validation and Guidance for Neurological Practice

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To (1) validate GAD65‐ELISA detection and quantification for type 1 diabetes mellitus and autoimmune neurological diagnoses, (2) correlate ELISA results (reference range < 5 IU/mL) with established radioimmunoprecipitation assay (RIA; ≤ 0.02 nmol/L), and (3) define ELISA clinical utility and pitfalls.
Andrew McKeon, Dana Olofson, Diana Anissian, Divyanshu Dubey, Eoin P. Flanagan, Max C. Herman, Sarosh R. Irani, Daniel H. Lachance, Maria A. Willrich, Sean Pittock, John R. Mills, Anastasia Zekeridou   +11 more
wiley   +1 more source

Evaluation of enzyme immunoassays in the diagnosis of camel (Camelus dromedarius) trypanosomiasis:a preliminary investigation [PDF]

, 1989
Three enzyme immunoassays were used for the serodiagnosis of Trypanosoma evansi in camels in the Sudan in order to evaluate their ability to discriminate between infected and non-infected animals.
Luckins, A G, Higgins, A, Jones, T W, Aitken, C G, Thrusfield, M V, Rae, P F   +5 more
core  

Enzyme-Linked Immunosorbent Assays for Cholera Serology [PDF]

Infection and Immunity, 1973
The enzyme-linked immunosorbent assay (ELISA) principle of Engvall and Perlmann, which employs antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was elaborated for use in cholera serology. Immunoglobulin class-specific determinations of primary binding titers of antibodies to cholera exotoxin and endotoxin were more sensitive than were ...
J, Holmgren, A M, Svennerholm
openaire   +2 more sources

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations [PDF]

, 1999
A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E ...
Deplazes, P., Thompson, R.C.A., Alther, P., Eckert, J., Tanner, I.   +4 more
core   +1 more source

Accuracy parameters of immunodiffusion and enzyme-linked immunosorbent assay tests. [PDF]

, 2015
DID:double agar gel immunodiffusion; ELISA: enzyme-linked immunosorbent assay; 1: antigen the Aspergillus fumigatus; 2: pool de antigens the A. fumigatus, A. flavus e A.
Lídia Raquel de Carvalho (557816), Rinaldo Poncio Mendes (365963), Tatiane Fernanda Sylvestre (553786), Daniela Vanessa Moris (557813), Priscila Zacarias de Azevedo (783296), Ricardo de Souza Cavalcante (553785), Maria Luiza Cotrim Sartor de Oliveira (513131)   +6 more
core   +1 more source

Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods Pesquisa epidemiologica da infecção por Trypanosoma cruzi no nordeste brasileiro utilizando-se diferentes métodos diagnósticos

Revista do Instituto de Medicina Tropical de São Paulo, 1991
A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence
José Figuerêdo-Silva, Yoshimasa Kaneda, Hiroshi Tachibana, Rieko Furushima, Seiki Tateno, Fernando Gomes Correia-Lima, Dalva Neves da Costa Bento   +6 more
doaj   +1 more source

Using a solid-phase variant of enzyme immunoassay for the etiological diagnosis of endogenous uveitis

Российский офтальмологический журнал, 2023
Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to diagnose infectious diseases, including ophthalmic ones. As a rule, however, investigators are only focused on differentiated detection of specific immunoglobulins (Ig) of varied ...
A. Yu. Kon’kova, E. S. Horowitz, T. V. Gavrilova, M. V. Chereshneva, E. V. Afanasievskaya   +4 more
doaj   +1 more source