Results 51 to 60 of about 394,097 (307)

Blood serum analysis: A modified sandwich enzyme-linked immunosorbent assay protocol

MethodsX, 2022
Blood serum analysis is a versatile tool used in diagnostics, in vivo research, and clinical studies. Enzyme-linked immunosorbent assay (ELISA) is a common method used to analyze blood serum cytokine levels; however, commercial kits are costly and not ...
Dunpeng Cai, Mikayla Fraunfelder, Shi-You Chen   +2 more
doaj   +1 more source

E2A selectively regulates TGF‐β–induced apoptosis in KRAS‐mutant non‐small cell lung cancer

Molecular Oncology, EarlyView.
Ability to induce apoptosis by TGF‐β is frequently lost in advanced lung adenocarcinoma despite intact TGF‐β signaling. We identify E2A as a mutant KRAS–dependent mediator of resistance to TGF‐β–induced apoptosis. TGF‐β induces E2A via SMAD3 in mutant KRAS cells, and E2A silencing restores apoptosis and enhances radiation response in cell lines ...
Sergei Chuikov, Shiva Krishna Katkam, Zhefan Wang, Venkateshwar G. Keshamouni   +3 more
wiley   +1 more source

Amphotericin B enzyme-linked immunosorbent assay [PDF]

Antimicrobial Agents and Chemotherapy, 1996
Our purpose was to develop and characterize an enzyme-linked immunosorbent assay (ELISA) which could measure the concentration of amphotericin B in serum. Amphotericin B was assayed by competition ELISA. Multiwell ELISA plates coated with amphotericin B (1.0 micrograms/ml) conjugated to bovine serum albumin were used to test replicates of serum samples
J D, Cleary, S W, Chapman, J, Deng, C J, Lobb   +3 more
openaire   +2 more sources

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

The enzyme-linked immunosorbent assay (ELISA): history, theory and application

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Enzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents.
Yu. N. Tarakanova, A. D. Dmitriev, D. A. Dmitriev, V. F. Lavrov, Yu. S. Massino, А. A. Pechelyulko, O. L. Segal   +6 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Enzyme Linked Immunosorbent Assay (ELISA) Technique Guideline

, 2021
ELISA (Enzyme-linked immunosorbent assay) is a technique used to assess the quantification of peptide, protein, antibody and hormone levels, based on the principle of antigen-antibody binding.
Patricia Wulandari, Hidayat, Rachmat
core   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods Pesquisa epidemiologica da infecção por Trypanosoma cruzi no nordeste brasileiro utilizando-se diferentes métodos diagnósticos

Revista do Instituto de Medicina Tropical de São Paulo, 1991
A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence
José Figuerêdo-Silva, Yoshimasa Kaneda, Hiroshi Tachibana, Rieko Furushima, Seiki Tateno, Fernando Gomes Correia-Lima, Dalva Neves da Costa Bento   +6 more
doaj   +1 more source

Using a solid-phase variant of enzyme immunoassay for the etiological diagnosis of endogenous uveitis

Российский офтальмологический журнал, 2023
Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to diagnose infectious diseases, including ophthalmic ones. As a rule, however, investigators are only focused on differentiated detection of specific immunoglobulins (Ig) of varied ...
A. Yu. Kon’kova, E. S. Horowitz, T. V. Gavrilova, M. V. Chereshneva, E. V. Afanasievskaya   +4 more
doaj   +1 more source