Results 51 to 60 of about 3,900,452 (305)

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Pressure: a novel tool for enzyme-linked immunosorbent assay procedure

BioTechniques, 2009
The enzyme-linked immunosorbent assay (ELISA) technique is the backbone of the diagnostic assay for detection of infectious and allergic diseases. Here, we demonstrate a unique ELISA method for the detection of an antigen or antibody, in which ELISA ...
Dileep Kumar Kannoujia, Pradip Nahar
doaj   +1 more source

Enzyme-linked immunosorbent assay using a virus type-specific peptide based on a subdomain of envelope protein e(rns) for serologic diagnosis of pestivirus infections in swine [PDF]

, 2001
Peptides deduced from the C-terminal end (residues 191 to 227) of pestivirus envelope protein Erns were used to develop enzyme-linked immunosorbent assays (ELISAs) to measure specifically antibodies against different types of pestiviruses.
Langedijk, J.P., Smit, J.A., de, Meloen, R.H., Middel, W.G., Kramps, J.A.   +4 more
core   +1 more source

Integrated PANoptosis Profiling Identifies Immunosuppressive Subtypes and a Prognostic Signature With Functional Validation of MLKL in Glioblastoma

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The prognosis of glioblastoma (GBM) remains highly unfavorable, largely due to high tumor heterogeneity and an immunosuppressive microenvironment. However, the functional role of PANoptosis in this context is poorly understood. Methods Patients were stratified via K‐means clustering. A risk score model was constructed using prognosis‐
Langfei Tian, Minghui Zhao, Yuanbo Hu, Kaiyue Wang, Haiguang Liu, Zetong Bai, Kebin Zheng   +6 more
wiley   +1 more source

DETECTION OF HUMAN BLOODSTAINS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) [PDF]

Acta Medica Iranica, 1982
The microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (ELISA) , usi ng alkaline phosphatase anti-human I gG conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed
GHOLAM-HOSSEIN EDRISSIAN, ABBAS HAFIZI   +1 more
doaj   +1 more source

The Comprehensive Live Cell‐Based Cytotoxicity Assay for Monitoring Disease Activity and Guiding Rescue Therapy in Acute Attacks of NMOSD

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Neuromyelitis optica spectrum disorder (NMOSD) is a devastating neurological disease that lacks serological biomarkers that can accurately reflect disease activity. We established a live cell‐based assay (LCBA) using serum with endogenous complement to quantify the overall cytotoxicity, offering a novel functional tool for monitoring
Xiaona Xu, Jie Ding, Yupeng Du, Guibao Luo, Jinming Li, Rui Liu, Xiaoyi Xu, Xiao Li, Minshu Li, Wei Jiang, Chunsheng Yang   +10 more
wiley   +1 more source

The enzyme-linked immunosorbent assay (ELISA): history, theory and application

Журнал микробиологии, эпидемиологии и иммунобиологии, 2019
Enzyme-linked immunosorbent assay (ELISA) play a significant role in the development of many important fields of biology and medicine, including the detection of the dangerous infections agents.
Yu. N. Tarakanova, A. D. Dmitriev, D. A. Dmitriev, V. F. Lavrov, Yu. S. Massino, А. A. Pechelyulko, O. L. Segal   +6 more
doaj   +1 more source

Amphotericin B enzyme-linked immunosorbent assay [PDF]

Antimicrobial Agents and Chemotherapy, 1996
Our purpose was to develop and characterize an enzyme-linked immunosorbent assay (ELISA) which could measure the concentration of amphotericin B in serum. Amphotericin B was assayed by competition ELISA. Multiwell ELISA plates coated with amphotericin B (1.0 micrograms/ml) conjugated to bovine serum albumin were used to test replicates of serum samples
J D, Cleary, S W, Chapman, J, Deng, C J, Lobb   +3 more
openaire   +2 more sources

Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium [PDF]

, 2003
A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E.
Sumption, Keith J, Paxton, Edith, Bell-Sakyi, Lesley   +2 more
core  

Investigation of Complement Activation and Cytokine Signatures in AQP4+ NMOSD, MOGAD, and MS

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Aquaporin‐4‐IgG seropositive neuromyelitis optica spectrum disorder (AQP4+ NMOSD), myelin oligodendrocyte glycoprotein antibody‐associated disease (MOGAD), and multiple sclerosis (MS) are pathophysiologically distinct neuroinflammatory disorders. Complement activation is a major mechanism of injury in AQP4+ NMOSD, whereas its role in
Angeliki G. Filippatou, Susana Dominguez‐Penuela, David Acero‐Garces, Georgios Gakis, Matthew D. Smith, Yasmin Resto, Maria Reyes‐Mantilla, Haiwen Chen, Isabella Hernandez, Yana Said, Andrea Salazar‐Camelo, Christina Huynh, Alessia Tassoni, Ted Yednock, Rick Artis, Yaisa Andrews‐Zwilling, Scott D. Newsome, Shiv Saidha, Pavan Bhargava, Ellen M. Mowry, Kathryn C. Fitzgerald, Peter A. Calabresi, Carlos A. Pardo, Elias S. Sotirchos   +23 more
wiley   +1 more source