Results 51 to 60 of about 395,671 (307)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA [PDF]

, 2005
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA).
Rudolf M. Lequin, Rudolf M Lequin
core   +1 more source

Pressure: a novel tool for enzyme-linked immunosorbent assay procedure

BioTechniques, 2009
The enzyme-linked immunosorbent assay (ELISA) technique is the backbone of the diagnostic assay for detection of infectious and allergic diseases. Here, we demonstrate a unique ELISA method for the detection of an antigen or antibody, in which ELISA ...
Dileep Kumar Kannoujia, Pradip Nahar
doaj   +1 more source

Time‐restricted feeding prior to Mycobacterium tuberculosis infection reduces tissue CD4+ T cells with limited impact on bacterial clearance

FEBS Open Bio, EarlyView.
Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta, Nidhi Yadav, Subhasmita Das, R. Rajendra Kumar Reddy, Nupur Sharma, Amol Ratnakar Suryawanshi, Jaswinder Singh Maras, Ranjan Kumar Nanda   +7 more
wiley   +1 more source

DETECTION OF HUMAN BLOODSTAINS BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) [PDF]

Acta Medica Iranica, 1982
The microplate method of e nzyme-l i nke d i rnmuno s o r be n t a s s ay (ELISA) , usi ng alkaline phosphatase anti-human I gG conj ugate , was modified and appl ied in t he dete c t i on of the human b lood specimens among the blood samples prep a r ed
GHOLAM-HOSSEIN EDRISSIAN, ABBAS HAFIZI   +1 more
doaj   +1 more source

Enzyme-linked immunosorbent assay for chlamydial antibodies [PDF]

Journal of Clinical Microbiology, 1977
An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The assay antigen produced in cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by ELISA. Paired
V J, Lewis, W L, Thacker, S H, Mitchell
openaire   +2 more sources

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium [PDF]

, 2003
A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E.
Sumption, Keith J, Paxton, Edith, Bell-Sakyi, Lesley   +2 more
core  

The Seroepidemiology of Haemophilus influenzae Type B Prior to Introduction of an Immunization Programme in Kathmandu, Nepal. [PDF]

, 2014
Haemophilus influenzae type b (Hib) is now recognized as an important pathogen in Asia. To evaluate disease susceptibility, and as a marker of Hib transmission before routine immunization was introduced in Kathmandu, 71 participants aged 7 months-77 ...
Neelam Adhikari, Thorson, SM, Clutterbuck, EA, Marshall, AS, Gautam Samir C., Adhikari, N, Shrestha, S, Barker, CI, Andrew J. Pollard (44971), Shrestha Shrijana, Barker Charlotte I. S., Pollard Andrew J., Kelly Dominic F., Elizabeth A Clutterbuck, Dominic F. Kelly (301751), Samir C Gautam, Adhikari Neelam, Charlotte I S Barker, Clutterbuck Elizabeth A., Pulickal Anoop S., Shrijana Shrestha, Kibwana, E, Dominic F Kelly, Andrew J Pollard, Marshall Andrew S. J., Pulickal, AS, Gautam, SC, Andrew S J Marshall, Anoop S. Pulickal (512430), Anoop S Pulickal, Thorson Stephen M., Charlotte I. S. Barker (512429), Samir C. Gautam (301746), Kibwana Elizabeth, Stephen M Thorson, Elizabeth Kibwana (512431), Kelly, DF, Stephen M. Thorson (512433), Pollard, AJ, Neelam Adhikari (301753), Pollard, Andrew, Elizabeth Kibwana, Andrew S. J. Marshall (512428), Shrijana Shrestha (512434), Elizabeth A. Clutterbuck (512432)   +44 more
core   +2 more sources