Results 41 to 50 of about 3,416,605 (304)

Enzyme-Linked Immunosorbent Assay and Serologic Responses to Pneumocystis jiroveci

Emerging Infectious Diseases, 2004
Seroepidemiologic studies of Pneumocystis pneumonia (PCP) in humans have been limited by inadequate reagents. We have developed an enzyme-linked immunosorbent assay (ELISA) using three overlapping recombinant fragments of the human Pneumocystis major ...
Kieran R. Daly, Judy Koch, Linda Levin, Peter D. Walzer   +3 more
doaj   +1 more source

Pleural effusion caused by Trichinella spiralis infection: two case reports

BMC Infectious Diseases, 2023
Background Trichinosis is a worldwide food-borne zoonotic parasitic disease, which is mainly obtained by ingesting undercooked meat containing infected larvae.
Zhen-zhen Pan, Miao-juan Zhu, Yu-qiong Rong, Jiong Yang   +3 more
doaj   +1 more source

Using a solid-phase variant of enzyme immunoassay for the etiological diagnosis of endogenous uveitis

Российский офтальмологический журнал, 2023
Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to diagnose infectious diseases, including ophthalmic ones. As a rule, however, investigators are only focused on differentiated detection of specific immunoglobulins (Ig) of varied ...
A. Yu. Kon’kova, E. S. Horowitz, T. V. Gavrilova, M. V. Chereshneva, E. V. Afanasievskaya   +4 more
doaj   +1 more source

Enzyme-Linked Immunosorbent Assay for Detection of Chitooligosaccharides

Bioscience, Biotechnology, and Biochemistry, 2000
In order to detect chitooligosaccharides (COS), an enzyme-linked immunosorbent assay (ELISA) was developed. A chitooligosaccharide mixture (COSM) conjugated to bovine serum albumin was used to immunize rabbits to produce an anti-COS polyclonal antibody.
KIM, Soon-Young, SHON, Dong-Hwa, LEE, Ke-Ho   +2 more
openaire   +2 more sources

Development of an enzyme‑linked immunosorbent assay for camptothecin

Molecular Medicine Reports, 2019
The use of camptothecin and its analogues has increased in clinical settings and in agriculture. Therefore, camptothecins and their derivatives, metabolites and degradation products are frequently found in the environment. Therefore, it is important to develop an ELISA for the quantification of camptothecins in human plasma, plants, animal tissues and ...
Yan, Liting, Nan, Xiang, Zhang, Cunzheng, Wang, Haifang, Huang, Xiaoyan, Hu, Jun, Liu, Yingqian   +6 more
openaire   +3 more sources

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Pressure: a novel tool for enzyme-linked immunosorbent assay procedure

BioTechniques, 2009
The enzyme-linked immunosorbent assay (ELISA) technique is the backbone of the diagnostic assay for detection of infectious and allergic diseases. Here, we demonstrate a unique ELISA method for the detection of an antigen or antibody, in which ELISA ...
Dileep Kumar Kannoujia, Pradip Nahar
doaj   +1 more source

Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods

, 2022
Kangaroos are considered to be an important reservoir of Q fever in Australia, although there is limited knowledge on the true prevalence and distribution of coxiellosis in Australian macropod populations.
Tolpinrud, A, Pas, An, Chaber, Anne-Lise, Pas, A, Stenos, John, Firestone, SM, Firestone, Simon M, Herbert, Catherine, Devlin, Joanne M., Devlin, JM, Herbert, C, Tolpinrud, Anita, Devlin, Joanne M, Dunowska, Magdalena, Firestone, Simon M., Stevenson, MA, Stevenson, Mark A., Dunowska, M, Stenos, J, Chaber, AL, Stevenson, Mark A   +20 more
core   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: Establishment of an efficient ELISA for antigen detection in feces [PDF]

, 1989
Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens.
Eichhorn, Werner, Czerny, C. P.
core   +1 more source