Results 21 to 30 of about 3,900,452 (305)

Enzyme-linked immunosorbent assay for chromogranin A.

open access: yesClinical Chemistry, 1989
Abstract This is an enzyme-linked immunosorbent assay (ELISA) for determining chromogranin A (CGA) with use of a monoclonal antibody. CGA was isolated from bovine chromaffin granules. The analytical ELISA procedure for bovine CGA was developed and optimized. Typical standard curves ranged from 500 pg to 500 ng of CGA.
Dillen, L.   +3 more
openaire   +3 more sources

Comparison between enzyme-linked immunosorbent assay and indirect immunofluorescence for detection of antineutrophil cytoplasmic antibodies

open access: yesEinstein (São Paulo), 2020
Objective To evaluate the performance of enzyme-linked immunosorbent assay and indirect immunofluorescence methods for the detection of antineutrophil cytoplasmic antibodies in a routine clinical laboratory setting. Methods A total of 227 samples were
Julia Miranda Menezes   +4 more
doaj   +2 more sources

Seroprevalence of Chikungunya (CHIK) Infection during Non Epidemic Periods in Chennai, Southern India [PDF]

open access: yesJournal of Clinical and Diagnostic Research, 2021
Introduction: Chikungunya (CHIK) infection has caused many outbreaks in India with more than 13 lac people affected by the disease. Epidemics of CHIK infection occur during post monsoon period when there is a high vector density.
Kaliyamoorthy Kayalvili   +3 more
doaj   +1 more source

Diagnosis of Cylindrocarpon destructans Using Enzyme-Linked Immunosorbent Assay

open access: yesResearch in Plant Disease, 2019
Cylindrocarpon destructans causes ginseng root rot and produces radicicol that has an antifungal effect. In this study, we developed a method to detect this fungus using enzyme-linked immunosorbent assay (ELISA). Secreted proteins of C.
Taiying Li   +8 more
doaj   +1 more source

Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis [PDF]

open access: yes, 2001
An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked ...
Abdoel, Theresia H   +10 more
core   +1 more source

Development of a Simple Pretreatment Immunoassay Based on an Organic Solvent-Tolerant Nanobody for the Detection of Carbofuran in Vegetable and Fruit Samples

open access: yesBiomolecules, 2019
Nanobodies are one-tenth the size of conventional antibodies and are naturally obtained from the atypical heavy-chain-only antibodies present in camelids.
Jin-ru Zhang   +10 more
doaj   +1 more source

Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay (ELISA [PDF]

open access: yes, 2005
This brief note addresses the historical background of the invention of the enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA).
Rudolf M. Lequin, Rudolf M Lequin
core   +1 more source

Using Enzyme-Linked Aptamer Assay to Detect Hepatitis B Surface Antigen in Comparison to Enzyme-Linked Immunosorbent Assay

open access: yesمجله دانشکده پزشکی اصفهان, 2018
Background: Clinical diagnostic kits for detecting hepatitis B are based on antibodies, and have inefficient sensitivity, stability, and cost. Therefore, researches about aptamers and using them instead of antibodies may make these defects up.
Hamidreza Mianesaz   +4 more
doaj   +1 more source

A membrane-based ELISA assay for the herbicide Isoproturon in soil samples [PDF]

open access: yes, 2012
A membrane based enzyme linked immunosorbent assay (MELISA) for the detection of a common herbicide, isoproturon is described. A heterogeneous competitive ELISA was the format chosen for isoproturon detection.
Baskeyfield, Damian E. H.   +7 more
core   +1 more source

Development of Immunoassay Based on Monoclonal Antibody Reacted with the Neonicotinoid Insecticides Clothianidin and Dinotefuran

open access: yesSensors, 2012
Enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody (MoAb) was developed for the neonicotinoid insecticide clothianidin. A new clothianidin hapten (3-[5-(3-methyl-2-nitroguanidinomethyl)-1,3-thiazol-2-ylthio] propionic acid) was ...
Seiji Iwasa   +4 more
doaj   +1 more source

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