Results 21 to 30 of about 2,890,302 (318)

Comparative Study of Single-stranded Oligonucleotides Secondary Structure Prediction Tools

BMC Bioinformatics, 2023
Background Single-stranded nucleic acids (ssNAs) have important biological roles and a high biotechnological potential linked to their ability to bind to numerous molecular targets. This depends on the different spatial conformations they can assume. The
Thomas Binet, Séverine Padiolleau-Lefèvre, Stéphane Octave, Bérangère Avalle, Irene Maffucci   +4 more
doaj   +1 more source

Enzyme Kinetics at High Enzyme Concentration

Bulletin of Mathematical Biology, 2000
We re-visit previous analyses of the classical Michaelis-Menten substrate-enzyme reaction and, with the aid of the reverse quasi-steady-state assumption, we challenge the approximation d[C]/dt approximately 0 for the basic enzyme reaction at high enzyme concentration.
Schnell, S, Maini, P
openaire   +3 more sources

The alkynyl-containing compounds from mushrooms and their biological activities

Natural Products and Bioprospecting, 2023
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi, Yingce Duan, Zhao-chen Li, Jin-ming Gao, Jianzhao Qi, Chengwei Liu   +5 more
doaj   +1 more source

Clinical Utility of SARS-CoV-2 Antibody Titer Multiplied by Binding Avidity of Receptor-Binding Domain (RBD) in Monitoring Protective Immunity and Clinical Severity

Viruses, 2023
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi, Takako Sawabuchi, Tetsuya Homma, Yosuke Fukuda, Hironori Sagara, Takeshi Kinjo, Kaori Fujita, Shigeru Suga, Takashi Kimoto, Satoko Sakai, Keiko Kameda, Hiroshi Kido   +11 more
doaj   +1 more source

ENZYMES AND ANTI-ENZYMES OF INFLAMMATORY EXUDATES [PDF]

Journal of Experimental Medicine, 1904
The serum of an inflammatory exudate has the power of inhibiting the action of proteolytic ferments contained in the leucocytes. This anti-enzymotic power is possessed by the blood serum from which it doubtless passes into the exudate. In the later stages of inflammation there is some diminution of this anti-enzymotic action.
openaire   +2 more sources

Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation

BMC Genomics, 2023
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang, Wu-Wei-Jie Yang, Ting-Ting Qiu   +2 more
doaj   +1 more source

Umami and saltiness enhancements of textured pea proteins by combining protease- and glutaminase-catalyzed reactions

Current Research in Food Science
Plant-based meat analogs (PBMAs) have attracted attention owing to their various advantages, however, their taste limits their application, requiring improvement of the umami and saltiness levels while meeting clean-label requirements.
Kiyota Sakai, Nickolas Broches, Keita Okuda, Masamichi Okada, Shotaro Yamaguchi   +4 more
doaj   +1 more source

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

Microbiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu, Shijing Xu, Liuwei Sun, Yiqiu Zhang, Jing Luo, Ralph Bock, Jiang Zhang   +6 more
doaj   +1 more source

Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

BMC Biotechnology, 2017
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu, Hisham Mohd Nooh, Siti Nurbaya Oslan, Abu Bakar Salleh   +3 more
doaj   +1 more source

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Microbial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn, Laura Kuschmierz, Nuka Benz, Patrick Lenz, Andreas Knapp, Karl-Erich Jaeger   +5 more
doaj   +1 more source