Results 31 to 40 of about 4,313,489 (338)

Clinical Utility of SARS-CoV-2 Antibody Titer Multiplied by Binding Avidity of Receptor-Binding Domain (RBD) in Monitoring Protective Immunity and Clinical Severity

open access: yesViruses, 2023
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi   +11 more
doaj   +1 more source

The alkynyl-containing compounds from mushrooms and their biological activities

open access: yesNatural Products and Bioprospecting, 2023
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi   +5 more
doaj   +1 more source

Enzymes as chaperones and chaperones as enzymes

open access: yesFEBS Letters, 1998
Chaperones and foldases are two groups of accessory proteins which assist maturation of nascent peptides into functional proteins in cells. Protein disulfide isomerase, a foldase, and ATP‐dependent proteases, responsible for degradation of misfolded proteins in cells, both have intrinsic chaperone activities.
Chen Lu Tsou, Chih-chen Wang
openaire   +3 more sources

Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation

open access: yesBMC Genomics, 2023
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang   +2 more
doaj   +1 more source

Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants [PDF]

open access: yes, 2005
The crystallization of three catalytically inactive mutants of penicillin Vacylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported.
Brannigan, J A   +6 more
core   +2 more sources

Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 [PDF]

open access: yes, 2005
A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43
A. Rahman, R   +7 more
core   +1 more source

Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

open access: yesBMC Biotechnology, 2017
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu   +3 more
doaj   +1 more source

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

open access: yesMicrobial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn   +5 more
doaj   +1 more source

Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]

open access: yes, 1985
The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina,   +8 more
core   +2 more sources

The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization

open access: yes, 2016
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
Bar-Even, Arren   +5 more
core   +3 more sources

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