Results 31 to 40 of about 4,423,700 (359)

Umami and saltiness enhancements of textured pea proteins by combining protease- and glutaminase-catalyzed reactions

open access: yesCurrent Research in Food Science
Plant-based meat analogs (PBMAs) have attracted attention owing to their various advantages, however, their taste limits their application, requiring improvement of the umami and saltiness levels while meeting clean-label requirements.
Kiyota Sakai   +4 more
doaj   +1 more source

Formation of Enzyme-Enzyme Heteroconjugates

open access: yesAgricultural and Biological Chemistry, 1984
Enzyme-enzyme heteroconjugates of phosphoglyceromutase, enolase and pyruvate kinase, which sequentially act to produce ATP in the glycolytic pathway, were constructed with a bifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The conjugates of phospho-glyceromutase-enolase, enolase-pyruvate kinase and phosphoglyceromutase-enolase ...
Katsuzumi Okumura   +3 more
openaire   +3 more sources

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

open access: yesMicrobiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu   +6 more
doaj   +1 more source

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

open access: yesFEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia

open access: yesBMC Genetics, 2020
Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected ...
Natalia Blazhko   +7 more
doaj   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

open access: yesFEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients

open access: yesFrontiers in Microbiology, 2016
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function.
Jie eYang   +8 more
doaj   +1 more source

Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

open access: yesJournal of Immunology, 1972
A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after
E. Engvall, P. Perlmann
semanticscholar   +1 more source

A stepwise emergence of evolution in the RNA world

open access: yesFEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 [PDF]

open access: yesPeerJ, 2016
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of ...
Wahhida Latip   +4 more
doaj   +2 more sources

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