Results 31 to 40 of about 4,313,489 (338)
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi+11 more
doaj +1 more source
The alkynyl-containing compounds from mushrooms and their biological activities
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi+5 more
doaj +1 more source
Enzymes as chaperones and chaperones as enzymes
Chaperones and foldases are two groups of accessory proteins which assist maturation of nascent peptides into functional proteins in cells. Protein disulfide isomerase, a foldase, and ATPādependent proteases, responsible for degradation of misfolded proteins in cells, both have intrinsic chaperone activities.
Chen Lu Tsou, Chih-chen Wang
openaire +3 more sources
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang+2 more
doaj +1 more source
Cloning, preparation and preliminary crystallographic studies of penicillin V acylase autoproteolytic processing mutants [PDF]
The crystallization of three catalytically inactive mutants of penicillin Vacylase (PVA) from Bacillus sphaericus in precursor and processed forms is reported.
Brannigan, J A+6 more
core +2 more sources
Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1 [PDF]
A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43
A. Rahman, R+7 more
core +1 more source
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu+3 more
doaj +1 more source
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn+5 more
doaj +1 more source
Cyclic nucleotide specificity of the activator and catalytic sites of a cGMP-stimulated cGMP phosphodiesterase from Dictyostelium discoideum [PDF]
The cellular slime mold Dictyostelium discoideum has an intracellular phosphodiesterase which specifically hydrolyzes cGMP. The enzyme is activated by low cGMP concentrations, and is involved in the reduction of chemoattractant-mediated elevations of ...
Baraniak, Janina,+8 more
core +2 more sources
The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
Bar-Even, Arren+5 more
core +3 more sources