Results 31 to 40 of about 6,346,353 (348)

Enhanced lipid extraction from unbroken microalgal cells using enzymes [PDF]

, 2015
The marine microalga Nannochloropsis sp. was chosen as a model organism to investigate the feasibility of using cell wall-degrading enzymes to enhance the recovery of intracellular lipids.
Familiari, Giuseppe, Lavecchia, Roberto, Maffei, Gianluca, Marra, Francesco, Miglietta, Selenia, Petrangeli, Alberto, Valente, Teodoro, Zuorro, Antonio   +7 more
core   +1 more source

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Microbial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn, Laura Kuschmierz, Nuka Benz, Patrick Lenz, Andreas Knapp, Karl-Erich Jaeger   +5 more
doaj   +1 more source

Pathway of human AS3MT arsenic methylation [PDF]

, 2014
A synthetic gene encoding human As(III) S-adenosylmethionine (SAM) methyltransferase (hAS3MT) was expressed, and the purified enzyme was characterized.
Dheeman, Dharmendra S., Packianathan, Charles, Pillai, Jitesh K., Rosen, Barry P.   +3 more
core   +2 more sources

Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus

Nature, 2003
Spike (S) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (SARS), associate with cellular receptors to mediate infection of their target cells. Here we identify a metallopeptidase, angiotensin-converting
Wenhui Li, M. J. Moore, N. Vasilieva, J. Sui, S. Wong, M. Berne, M. Somasundaran, J. Sullivan, K. Luzuriaga, T. Greenough, H. Choe, M. Farzan   +11 more
semanticscholar   +1 more source

Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

BMC Biotechnology, 2017
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu, Hisham Mohd Nooh, Siti Nurbaya Oslan, Abu Bakar Salleh   +3 more
doaj   +1 more source

Umami and saltiness enhancements of textured pea proteins by combining protease- and glutaminase-catalyzed reactions

Current Research in Food Science
Plant-based meat analogs (PBMAs) have attracted attention owing to their various advantages, however, their taste limits their application, requiring improvement of the umami and saltiness levels while meeting clean-label requirements.
Kiyota Sakai, Nickolas Broches, Keita Okuda, Masamichi Okada, Shotaro Yamaguchi   +4 more
doaj   +1 more source

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

Microbiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu, Shijing Xu, Liuwei Sun, Yiqiu Zhang, Jing Luo, Ralph Bock, Jiang Zhang   +6 more
doaj   +1 more source

Studies on the changes in protein fluorescence and enzymic activity of aspartate aminotransferase on binding of pyridoxal 5'-Phosphate [PDF]

, 1974
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3.
Evans, RW, Holbrook, J
core   +2 more sources

Mechanisms and kinetic assays of aminoacyl‐tRNA synthetases

FEBS Letters, EarlyView.
Accurate protein synthesis is crucial for life. The key players are aminoacyl‐tRNA synthetases (AARSs), which read the genetic code by pairing cognate amino acids and tRNAs. AARSs establish high amino acid selectivity by employing physicochemical limits in molecular recognition.
Igor Zivkovic, Morana Dulic, Ita Gruic‐Sovulj   +2 more
wiley   +1 more source

Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia

BMC Genetics, 2020
Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected ...
Natalia Blazhko, Sultan Vyshegurov, Alexander Donchenko, Kirill Shatokhin, Valeria Ryabinina, Kirill Plotnikov, Alevtina Khodakova, Sergey Pashkovskiy   +7 more
doaj   +1 more source