Results 31 to 40 of about 3,915,254 (313)

Master curve of boosted diffusion for ten catalytic enzymes [PDF]

open access: yes, 2020
Molecular agitation more rapid than thermal Brownian motion is reported for cellular environments, motor proteins, synthetic molecular motors, enzymes, and common chemical reactions, yet that chemical activity couples to molecular motion contrasts with generations of accumulated knowledge about diffusion at equilibrium. To test the limits of this idea,
arxiv   +1 more source

Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

open access: yesBMC Biotechnology, 2017
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu   +3 more
doaj   +1 more source

Genome-wide study of Cerrena unicolor 87613 laccase gene family and their mode prediction in association with substrate oxidation

open access: yesBMC Genomics, 2023
Background Laccases are green biocatalysts with wide industrial applications. The study of efficient and specific laccase producers remains a priority.
Long-Bin Zhang   +2 more
doaj   +1 more source

Direct Single Molecule Imaging of Enhanced Enzyme Diffusion [PDF]

open access: yesPhys. Rev. Lett. 123, 128101 (2019), 2018
Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence intensity signal to measure the diffusion coefficient of particles, has typically been employed in most of the prior ...
arxiv   +1 more source

Enzyme-enriched condensates show self-propulsion, positioning, and coexistence [PDF]

open access: yes, 2023
Enzyme-enriched condensates can organize the spatial distribution of their substrates by catalyzing non-equilibrium reactions. Conversely, an inhomogeneous substrate distribution induces enzyme fluxes through substrate-enzyme interactions. We find that condensates move towards the center of a confining domain when this feedback is weak.
arxiv   +1 more source

Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients

open access: yesFrontiers in Microbiology, 2016
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function.
Jie eYang   +8 more
doaj   +1 more source

A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

open access: yesCommunications Biology, 2021
Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells.
Shengchen Wang   +7 more
doaj   +1 more source

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

open access: yesMarine Drugs, 2021
Penicillium oxalicum k10 isolated from soil revealed the hydrolyzing ability of shrimp chitin and antifungal activity against Sclerotinia sclerotiorum. The k10 chitinase was produced from a powder chitin-containing medium and purified by ammonium sulfate
Xing-Huan Xie   +4 more
doaj   +1 more source

Transaminase Enzyme and Liver Histological Profile of Mice Administered Extract of Pegagan (Centella Asiatica (L.) Urban) [PDF]

open access: yes, 2011
The purpose of this study was to determine whether pegagan caused toxic effects on the liver. This study used completely randomized design with four treatments, ie 125, 200, 275 mg/kg BW and control, each treatment consisting of six replicates. Variables
Adi, T. T. (T)   +2 more
core  

FoxO1 signaling in B cell malignancies and its therapeutic targeting

open access: yesFEBS Letters, EarlyView.
FoxO1 has context‐specific tumor suppressor or oncogenic character in myeloid and B cell malignancies. This includes tumor‐promoting properties such as stemness maintenance and DNA damage tolerance in acute leukemias, or regulation of cell proliferation and survival, or migration in mature B cell malignancies.
Krystof Hlavac   +3 more
wiley   +1 more source

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