Results 51 to 60 of about 6,423,741 (359)

Parameters necessary to define an immobilized enzyme preparation

Process Biochemistry, 2020
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation
J. Boudrant, J. Woodley, R. Fernández-Lafuente   +2 more
semanticscholar   +1 more source

Parallel versus off-pathway Michaelis-Menten mechanism for single-enzyme kinetics of a fluctuating enzyme

, 2015
Recent fluorescence spectroscopy measurements of the turnover time distribution of single-enzyme turnover kinetics of $\beta$-galactosidase provide evidence of Michaelis-Menten kinetics at low substrate concentration.
Dua, Arti, Kumar, Ashutosh, Maity, Hiranmay   +2 more
core   +2 more sources

Umami and saltiness enhancements of textured pea proteins by combining protease- and glutaminase-catalyzed reactions

Current Research in Food Science
Plant-based meat analogs (PBMAs) have attracted attention owing to their various advantages, however, their taste limits their application, requiring improvement of the umami and saltiness levels while meeting clean-label requirements.
Kiyota Sakai, Nickolas Broches, Keita Okuda, Masamichi Okada, Shotaro Yamaguchi   +4 more
doaj   +1 more source

Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology

BMC Biotechnology, 2017
Background Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the
Mary Ladidi Abu, Hisham Mohd Nooh, Siti Nurbaya Oslan, Abu Bakar Salleh   +3 more
doaj   +1 more source

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

Microbial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn, Laura Kuschmierz, Nuka Benz, Patrick Lenz, Andreas Knapp, Karl-Erich Jaeger   +5 more
doaj   +1 more source

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

Microbiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu, Shijing Xu, Liuwei Sun, Yiqiu Zhang, Jing Luo, Ralph Bock, Jiang Zhang   +6 more
doaj   +1 more source

Personalized Selumetinib Dosing in Pediatric Neurofibromatosis Type 1: Insights From a Pilot Therapeutic Drug Monitoring Study

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Objective To evaluate selumetinib exposure using therapeutic drug monitoring (TDM) in pediatric patients with neurofibromatosis type 1 (NF1) and plexiform neurofibromas (PN), assess interpatient pharmacokinetic variability, and explore the relationship between drug exposure, clinical response, and adverse effects.
Janka Kovács, Zoltán Köllő, Balázs Gellért Karvaly, Mahmoud Obeidat, Rita Nagy, Péter Hegyi, Brigitta Teutsch, Eszter Tuboly, Miklós Garami   +8 more
wiley   +1 more source

Transaminase Enzyme and Liver Histological Profile of Mice Administered Extract of Pegagan (Centella Asiatica (L.) Urban) [PDF]

, 2011
The purpose of this study was to determine whether pegagan caused toxic effects on the liver. This study used completely randomized design with four treatments, ie 125, 200, 275 mg/kg BW and control, each treatment consisting of six replicates. Variables
Adi, T. T. (T), Kiptiyah, K. (Kiptiyah), Muchtaromah, B. (B)   +2 more
core  

Sesquiterpene synthases from Inonotus obliquus: functional characterization and β-farnesene production

Mycology
The medicinal mushroom Inonotus obliquus is renowned for producing a wide array of pharmacologically active terpenoids, yet the enzymatic basis for this diversity remains partially elusive.
Yingce Duan, Jiaxuan Liu, Zheyong Xue, Pengchao Wang, Jie Zhang, Chengwei Liu   +5 more
doaj   +1 more source

Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Journal of Immunology, 1972
A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after
E. Engvall, P. Perlmann
semanticscholar   +1 more source