Results 51 to 60 of about 3,151,868 (313)

Enzyme-enzyme co-abundance analysis.

, 2017
(a) Enzyme-enzyme co-abundance network. Nodes and edges indicate enzymes and their correlations. Positive (r > 0.6) and negative (r < -0.6) correlations were indicated as red and blue, respectively.
Atsumi Tomita (3797761), Shunsuke Nishino (2554585), Hiroshi Shimizu (76745), Fumio Matsuda (230101), Syohei Kinoshita (3797758)   +4 more
core   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

From sequence to enzyme mechanism using multi-label machine learning [PDF]

, 2014
Background: In this work we predict enzyme function at the level of chemical mechanism, providing a finer granularity of annotation than traditional Enzyme Commission (EC) classes.
De Ferrari, Luna; id_orcid, De Ferrari, L., John BO Mitchell, Luna De Ferrari, Mitchell, J.B O., Mitchell, John B O   +5 more
core   +1 more source

Chelerythrine-Mediated Growth Inhibition and Resistance Mechanism in Bacillus tropicus

Microorganisms
Chelerythrine (CHE) is a naturally occurring benzophenanthridine alkaloid obtained from plants such as Chelidonium majus L. It has received notable attention in pharmacology and microbial control because of its broad-spectrum activity and marked anti ...
Jueyu Wang, Hongxia Wan, Wenqi Chai, Daizong Cui, Min Zhao   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Enzyme-substrate interaction modeling strategies.

, 2022
(A) Current machine learning-directed evolution strategies, which involve design-build-test-model-learn cycles measuring protein variant activity on a single substrate of interest.
Samuel Goldman (1699888), Connor W. Coley (1496653), Ria Das (12072859), Kevin K. Yang (4528963)   +3 more
core   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

The molecular basis of the effect of temperature on enzyme activity [PDF]

, 2010
Experimental data show that the effect of temperature on enzymes cannot be adequately explained in terms of a two-state model based on increases in activity and denaturation.
Oudshoorn, Matthew Leslie, Kelly, S.M., Price, N.C., Weinberg, C.S., Monk, C.R., Oudshoorn, M.L., Monk, Colin R., Danson, Michael J., Price, Nicholas C., Danson, M.J., Lee, Charles Kai-Wu, Peterson, Michelle E., Lee, C.K., Peterson, M.E., Weinberg, Cristina S., Daniel, R.M., Daniel, Roy M., Kelly, Sharon M.   +17 more
core   +1 more source

Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients

Frontiers in Microbiology, 2016
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function.
Jie eYang, Jie eYang, Guozeng eWang, Guozeng eWang, Tzi Bun eNg, Juan eLin, Juan eLin, Xiuyun eYe, Xiuyun eYe   +8 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source