Results 61 to 70 of about 3,151,868 (313)

Antimicrobial potential of the combination of fulvic acid and Trichoderma asperellum against rice pathogens

Biological Control
In this study, fluvic acid was prepared from sludge waste. The prepared fulvic acid had almost the same chemical structure as the standard compound expect that it contained a slightly more functional groups, which increased stability and biological ...
Xinyi Hou, Daizong Cui, Jinming Yang, Zhenghao Fang, Min Zhao   +4 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia

BMC Genetics, 2020
Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected ...
Natalia Blazhko, Sultan Vyshegurov, Alexander Donchenko, Kirill Shatokhin, Valeria Ryabinina, Kirill Plotnikov, Alevtina Khodakova, Sergey Pashkovskiy   +7 more
doaj   +1 more source

Expression and characterization of thermotolerant lipase with broad pH profiles isolated from an Antarctic Pseudomonas sp strain AMS3 [PDF]

PeerJ, 2016
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of ...
Wahhida Latip, Raja Noor Zaliha Raja Abd Rahman, Adam Thean Chor Leow, Fairolniza Mohd Shariff, Mohd Shukuri Mohamad Ali   +4 more
doaj   +2 more sources

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Enzyme activity and dynamics in near-anhydrous conditions [PDF]

, 2009
Water is widely assumed to be essential for life 1, although the exact molecular basis of this requirement is unclear 2-4. Water facilitates protein motions 5-9 and although enzyme activity has been demonstrated at low hydrations in organic solvents 10 ...
John Finney, Moeava Tehei, Vandana Kurkal-Siebert, Jeremy Smith, Murielle Lopez, Rachel Dunn, Roy Daniel   +6 more
core  

Phage display-derived inhibitor of the essential cell wall biosynthesis enzyme MurF [PDF]

, 2008
Background To develop antibacterial agents having novel modes of action against bacterial cell wall biosynthesis, we targeted the essential MurF enzyme of the antibiotic resistant pathogen Pseudomonas aeruginosa.
Sanschagrin, François, Tom Clarke, Catherine Paradis-Bleau, Blewett, Ann, Roger C Levesque, Clark, Tom, Paradis-Bleau , Catherine, Ann Blewett, Adrian Lloyd, Levesque, Roger C., Lloyd, Adrian, François Sanschagrin, Timothy DH Bugg, Bugg, Tim   +13 more
core   +1 more source