Results 61 to 70 of about 4,423,700 (359)
Metabolic Optimization by Enzyme-Enzyme and Enzyme-Cytoskeleton Associations [PDF]
, 2012 Probably enzymes are not dispersed in the cytoplasm, but are bound to each other and to specific cytoskeleton proteins. Associations result in substrate channeling from one enzyme to another. Multienzymatic complexes, or metabolons have been detected in glycolysis, the Krebs cycle and oxidative phosphorylation.
Adela Mújica +7 more
openaire +3 more sources
Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells
FEBS Letters, EarlyView.Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan +2 more
wiley +1 more source
The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis
Microbial Cell Factories, 2021 Background Bacillus subtilis is one of the most important microorganisms for recombinant protein production. It possesses the GRAS (generally recognized as safe) status and a potent protein secretion capacity.
Patrick Lenz +9 more
doaj +1 more source
FEBS Letters, EarlyView.C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi +5 more
wiley +1 more source
FEBS Letters, EarlyView.The Arabidopsis mutants hls1 hlh1 and amp1 lamp1 exhibit pleiotropic developmental phenotypes. Although the functions of the causative genes remain unclear, they act in the same genetic pathway and are thought to generate non‐cell‐autonomous signals.
Takashi Nobusawa, Makoto Kusaba
wiley +1 more source
IUCrJ, 2020 The enzyme carbonic anhydrase binds its zinc ion by three histidine residues in a similar manner to the way copper is bound to nitrite reductase. This remote similarity has now been shown to be real [Andring et al. (2020). IUCrJ, 7, 287-293]. A carbonic anhydrase with two bound copper ions is also a nitrite reductase.
openaire +4 more sources
FEBS Letters, EarlyView.Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile +8 more
wiley +1 more source
Structure of the plastic-degrading Ideonella sakaiensis MHETase bound to a substrate
Nature Communications, 2019 Plastic polymer PET degrading enzymes are of great interest for achieving sustainable plastics recycling. Here, the authors present the crystal structures of the plastic degrading bacterial enzymes PETase, MHETase in its apo-form and MHETase bound to a ...
Gottfried J. Palm +9 more
doaj +1 more source
Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes
FEBS Letters, EarlyView.Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley +1 more source
Machine Learning in Enzyme Engineering
ACS Catalysis, 2019 Enzyme engineering plays a central role in developing efficient biocatalysts for biotechnology, biomedicine, and life sciences.
S. Mazurenko, Z. Prokop, J. Damborský
semanticscholar +1 more source