Characterization of α- Amylase Produced from a Local Isolate of Bacillus licheniformis R5 [PDF]
This research is conducted at the College of Agriculture, University of Baghdad in 2004. The characterization of the purified enzyme shows that the molecular weight of the enzyme produced from the bacteria is 56.000 Daltons as it was determined by gel ...
Rana Abdullah Hussein +1 more
doaj +1 more source
Enzyme activity below the dynamical transition at 220K [PDF]
Enzyme activity requires the activation of anharmonic motions, such as jumps between potential energy wells. However, in general, the forms and time scales of the functionally important anharmonic dynamics coupled to motion along the reaction coordinate ...
Smith, Jeremy C. +12 more
core +1 more source
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source
Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets. [PDF]
As shown by transgenic mouse models and by using phosphodiesterase 3 (PDE3) inhibitors, PDE3B has an important role in the regulation of insulin secretion in pancreatic β-cells. However, very little is known about the regulation of the enzyme.
Emilia Heimann +5 more
doaj +1 more source
Sensor potency of the moonlighting enzyme-decorated cytoskeleton [PDF]
Background: There is extensive evidence for the interaction of metabolic enzymes with the eukaryotic cytoskeleton. The significance of these interactions is far from clear.
Ripoll, Camille +5 more
core +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Determination of enzyme activities with the enzyme thermistor unit [PDF]
Practically all enzyrnic reactions can be followed by calorimetry. Important advantages of this technique are that the optical properties of the sample do not interfere with the measurements and that no auxiliary enzymic reactions are required such as those leading to changes in optical absorption that can be followed spectrophotometrically.
Danielsson, Bengt, Mosbach, Klaus
openaire +2 more sources
An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg +14 more
wiley +1 more source
A comparative study of enzyme immobilization strategies for multi-walled carbon nanotube glucose biosensors [PDF]
This work addresses the comparison of different strategies for improving biosensor performance using nanomaterials. Glucose biosensors based on commonly applied enzyme immobilization approaches, including sol-gel encapsulation approaches and ...
Claussen, Jonathan C. +17 more
core +1 more source
Discerning protein pools by selective staining with self‐labeling tags
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley +1 more source

